MicroRNA 21 and microRNA 10b: early diagnostic biomarkers of breast cancer in Egyptian females

被引:8
作者
Ali, Mai [1 ]
El Gayar, Dina [1 ]
Hany, Normeen [1 ]
Ezzat, Abdel Hamid [2 ]
Zeyada, Reham [1 ]
机构
[1] Cairo Univ, Fac Med, Cairo, Egypt
[2] Natl Canc Inst, Cairo, Egypt
关键词
miR21; miR10b; Breast cancer; Noninvasive biomarkers; Early detection; MIR-21;
D O I
10.1186/s43046-022-00115-6
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Breast cancer (BC) is one of the most prevalent cancers in developing and developed countries among women worldwide. Mammography is one of the superior methods for BC detection, but it carries up to 20% false-negative results, especially in early cases. Histological examination of tissue biopsies and fine-needle aspiration cytology are invasive techniques. Hence, minimally invasive markers are needed for the improved detection of BC. micro-RNAs, small, noncoding, single-stranded RNAs functioning as tumor suppressor genes or oncogenes, are attractive biomarkers for early detection. This study aimed to examine the serum levels of miR21 and miR10b in patients with BC especially in the early stages compared to healthy controls to evaluate their potential use as BC biomarkers. Methods: This study included 90 females who were divided into two groups. Group I included 70 patients with BC and was subdivided into group la with 40 nonmetastatic BC patients and group Ib with 30 metastatic BC patients. Group II included 20 apparently healthy females as a control group. Serum miR21 and miR10b as biomarkers and miR16 as a housekeeping gene were evaluated using real-time polymerase chain reaction. Results: The median levels of miR10b and miR21 were statistically significantly upregulated in the sera of patients with BC compared to healthy controls (P = 0.001). Receiver operating characteristic curve analyses demonstrated that serum levels of miR10b and miR21 were useful biomarkers for distinguishing between patients with BC and the con-trol group, with an area under the curve (AUC) of 0.991 with 97.1% sensitivity and 100% specificity at a cutoff of 3.1 for miR10b and an AUC of 0.965 with 95.7% sensitivity and 85% specificity at a cutoff of 1.7 for miR21. Regarding the early stages of BC, the median levels of the fold change of serum miR21 and miR10b were statistically significantly higher in patients with BC (stages I and IIa) than in the control group (P < 0.001). Conclusions: Both miR21 and miR10b have valuable diagnostic roles in detecting the early stages of BC.
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页数:10
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共 22 条
[1]  
Allam M F, 2012, J Prev Med Hyg, V53, P195
[2]   MicroRNAs as novel endogenous targets for regulation and therapeutic treatments [J].
Cha, Wenzhang ;
Fan, Rengen ;
Miao, Yufeng ;
Zhou, Yong ;
Qin, Chenglin ;
Shan, Xiangxiang ;
Wan, Xinqiang ;
Cui, Ting .
MEDCHEMCOMM, 2018, 9 (03) :396-408
[3]   Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012 [J].
Ferlay, Jacques ;
Soerjomataram, Isabelle ;
Dikshit, Rajesh ;
Eser, Sultan ;
Mathers, Colin ;
Rebelo, Marise ;
Parkin, Donald Maxwell ;
Forman, David ;
Bray, Freddie .
INTERNATIONAL JOURNAL OF CANCER, 2015, 136 (05) :E359-E386
[4]   Clinical significance of serum miR-21 in breast cancer compared with CA153 and CEA [J].
Gao, Jianjian ;
Zhang, Qingyun ;
Xu, Jianjun ;
Guo, Lijuan ;
Li, Xuefeng .
CHINESE JOURNAL OF CANCER RESEARCH, 2013, 25 (06) :743-748
[5]   Recall and Cancer Detection Rates for Screening Mammography: Finding the Sweet Spot [J].
Grabler, Paula ;
Sighoko, Dominique ;
Wang, Lilian ;
Allgood, Kristi ;
Ansell, David .
AMERICAN JOURNAL OF ROENTGENOLOGY, 2017, 208 (01) :208-213
[6]   Cancer Incidence in Egypt: Results of the National Population-Based Cancer Registry Program [J].
Ibrahim, Amal S. ;
Khaled, Hussein M. ;
Mikhail, Nabiel N. H. ;
Baraka, Hoda ;
Kamel, Hossam .
JOURNAL OF CANCER EPIDEMIOLOGY, 2014, 2014
[7]   Annual Report to the Nation on the Status of Cancer, 1975-2011, Featuring Incidence of Breast Cancer Subtypes by Race/Ethnicity, Poverty, and State [J].
Kohler, Betsy A. ;
Sherman, Recinda L. ;
Howlader, Nadia ;
Jemal, Ahmedin ;
Ryerson, A. Blythe ;
Henry, Kevin A. ;
Boscoe, Francis P. ;
Cronin, Kathleen A. ;
Lake, Andrew ;
Noone, Anne-Michelle ;
Henley, S. Jane ;
Eheman, Christie R. ;
Anderson, Robert N. ;
Penberthy, Lynne .
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2015, 107 (06)
[8]   MicroRNA biogenesis pathways in cancer [J].
Lin, Shuibin ;
Gregory, Richard I. .
NATURE REVIEWS CANCER, 2015, 15 (06) :321-333
[9]   Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].
Livak, KJ ;
Schmittgen, TD .
METHODS, 2001, 25 (04) :402-408
[10]   Role of miR-10b in breast cancer metastasis [J].
Ma, Li .
BREAST CANCER RESEARCH, 2010, 12 (05)