Smart antimicrobial efficacy employing pH-sensitive ZnO-doped diamond-like carbon coatings

被引：26

作者：

Buchegger, Sascha ^{[1
,4
]}

Kamenac, Andrej ^{[1
,4
]}

Fuchs, Sven ^{[1
]}

Herrmann, Rudolf ^{[1
]}

Houdek, Pia ^{[2
]}

Gorzelanny, Christian ^{[2
]}

Obermeier, Andreas ^{[3
]}

Heller, Stephan ^{[3
]}

Burgkart, Rainer ^{[3
]}

Stritzker, Bernd ^{[1
]}

Wixforth, Achim ^{[1
,4
]}

Westerhausen, Christoph ^{[1
,4
,5
]}

机构：

[1] Univ Augsburg, Chair Expt Phys 1, D-86159 Augsburg, Germany

[2] Univ Hosp Hamburg Eppendorf, Dept Dermatol & Venereol, D-20246 Hamburg, Germany

[3] Tech Univ Munich, Sch Med, Clin Orthoped & Sportorthoped, D-81675 Munich, Germany

[4] Ludwig Maximilians Univ Munchen, Ctr NanoSci CeNS, D-80799 Munich, Germany

[5] Univ Augsburg, Chair Physiol, D-86159 Augsburg, Germany

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

ANTIBACTERIAL EFFICACY; NANOPARTICLES; HYDROGELS; RELEASE; RAT;

D O I：

10.1038/s41598-019-53521-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

One of the main challenges in endoprosthesis surgeries are implant-associated infections and aseptic-loosenings, caused by wear debris. To combat these problems, the requirements to surfaces of endoprostheses are wear-resistance, low cytotoxicity and antimicrobial efficacy. We here present antimicrobial coatings with a smart, adaptive release of metal ions in case of infection, based on ZnO-nanoparticles embedded in diamond-like carbon (DLC). The Zn2+ ion release of these coatings in aqueous environments reacts and adapts smartly on inflammations accompanied by acidosis. Moreover, we show that this increased ion release comes along with an increased toxicity to fibroblastic cells (L929) and bacteria (Staphylococcus aureus subsp. aureus, resistant to methicillin and oxacillin. (ATCC 43300, MRSA) and Staphylococcus epidermidis (ATCC 35984, S. epidermidis). Interestingly, the antimicrobial effect and the cytotoxicity of the coatings increase with a reduction of the pH value from 7.4 to 6.4, but not further to pH 5.4.

引用

页数：10

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