Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels

被引：66

作者：

Alharbi, Naif Khalaf ^{[1
,2
]}

Qasim, Ibrahim ^{[3
]}

Almasoud, Abdulrahman ^{[1
]}

Aljami, Haya A. ^{[1
]}

Alenazi, Mohamed W. ^{[1
]}

Alhafufi, Ali ^{[3
]}

Aldibasi, Omar S. ^{[4
]}

Hashem, Anwar M. ^{[5
,6
]}

Kasem, Samy ^{[3
,7
]}

Albrahim, Raed ^{[3
]}

Aldubaib, Musaad ^{[8
]}

Almansour, Ali ^{[3
]}

Temperton, Nigel J. ^{[9
]}

Kupke, Alexandra ^{[10
,12
]}

Becker, Stephan ^{[10
,12
]}

Abu-obaidah, Ali ^{[3
]}

Alkarar, Ali ^{[3
]}

Yoon, In-Kyu ^{[11
]}

Azhar, Esam ^{[13
,14
]}

Lambe, Teresa ^{[15
]}

Bayoumi, Faisal ^{[3
]}

Aldowerij, Ali ^{[3
]}

Ibrahim, Osman H. ^{[3
]}

Gilbert, Sarah C. ^{[15
]}

Balkhy, Hanan H. ^{[1
,2
,16
]}

机构：

[1] King Abdullah Int Med Res Ctr, Dept Infect Dis Res, Riyadh, Saudi Arabia

[2] King Saud bin Abdulaziz Univ Hlth Sci, Riyadh, Saudi Arabia

[3] MEWA, Riyadh, Saudi Arabia

[4] King Abdullah Int Med Res Ctr, Dept Bioinformat & Biostat, Riyadh, Saudi Arabia

[5] King Abdulaziz Univ, Dept Med Microbiol & Parasitol, Fac Med, Jeddah, Saudi Arabia

[6] King Abdulaziz Univ, Vaccines & Immunotherapy Unit, King Fahd Med Res Ctr, Jeddah, Saudi Arabia

[7] Kafrelsheikh Univ, Dept Virol, Fac Vet Med, El Geish St, Kafrelsheikh 33516, Egypt

[8] Qassim Univ, Coll Agr & Vet Med, Qasim, Saudi Arabia

[9] Univ Kent, Medway Sch Pharm, Viral Pseudotype Unit, Chatham ME4 4TB, Kent, England

[10] Philipps Univ Marburg, Inst Virol, Marburg, Germany

[11] Int Vaccine Inst, Seoul, South Korea

[12] German Ctr Infect Res DZIF, Partner Site Giessen Marburg Langen, Munich, Germany

[13] King Abdulaziz Univ, Fac Appl Med Sci, Dept Med Lab Technol, Jeddah, Saudi Arabia

[14] King Abdulaziz Univ, Special Infect Agents Unit, King Fahd Med Res Ctr, Jeddah, Saudi Arabia

[15] Univ Oxford, Jenner Inst, Oxford OX3 7DQ, England

[16] Minist Natl Guard Hlth Affairs, Dept Infect Prevent & Control, Riyadh, Saudi Arabia

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

RESPIRATORY SYNDROME CORONAVIRUS; COV INFECTION; NEUTRALIZING ANTIBODIES; TRANSMISSION; PREVALENCE; RECEPTOR; AFRICA;

D O I：

10.1038/s41598-019-52730-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MERS-CoV seronegative and seropositive camels received a single intramuscular dose of ChAdOx1 MERS, a replication-deficient adenoviral vectored vaccine expressing MERS-CoV spike protein, with further groups receiving control vaccinations. Infectious camels with active naturally acquired MERSCoV infection, were co-housed with the vaccinated camels at a ratio of 1:2 (infected:vaccinated); nasal discharge and virus titres were monitored for 14 days. Overall, the vaccination reduced virus shedding and nasal discharge (p = 0.0059 and p = 0.0274, respectively). Antibody responses in seropositive camels were enhancedby the vaccine; these camels had a higher average age than seronegative. Older seronegative camels responded more strongly to vaccination than younger animals; and neutralising antibodies were detected in nasal swabs. Further work is required to optimise vaccine regimens for younger seronegative camels.

引用

页数：11

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