Effect of the aminosteroid U73122 on prostaglandin E2 production in a murine clonal osteoblast-like cell line, MC3T3-E1

被引：1

作者：

Leis, HJ ^{[1
]}

Köfeler, H ^{[1
]}

Raspotnig, G ^{[1
]}

Zach, D ^{[1
]}

Fauler, G ^{[1
]}

Windischhofer, W ^{[1
]}

机构：

[1] Graz Univ, Childrens Hosp, Dept Biochem Anal & Mass Spectrometry, A-8036 Graz, Austria

来源：

BIOCHEMICAL PHARMACOLOGY | 2000年 / 60卷 / 07期

关键词：

U73122; phospholipase C; osteoblast;

D O I：

10.1016/S0006-2952(00)00401-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Prostaglandin E-2 production stimulated by various agents (arachidonic acid, prostaglandin F-2 alpha, ionomycin, the calcium ionophore A23187, and melittin) was investigated after pretreatment of murine osteoblast-like MC3T3-E1 cells with the putative phospholipase C blocker, U73122. The aminosteroid dose dependently inhibited prostaglandin E-2 production induced by all agonists, except arachidonic acid. The results suggest an inhibitory role of U73122 on phospholipase A(2) activity or activation BIOCHEM PHARMACOL 60;7:899-904, 2000. (C) 2000 Elsevier Science Inc.

引用

页码：899 / 904

页数：6

共 31 条

[1] U73122 and U73343 inhibit receptor-mediated phospholipase D activation downstream of phospholipase C in CHO cells [J].

Bosch, RR ;

Patel, AMP ;

Van Emst-de Vries, SE ;

Smeets, RLL ;

De Pont, JJHHM ;

Willems, PHGM .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1998, 346 (2-3) :345-351

[2]

BURCH RM, 1988, J BIOL CHEM, V263, P4764

[3]

CHAN J, 1991, J PHARMACOL EXP THER, V258, P524

[4]

DEMOEL MP, 1995, EUR J BIOCHEM, V234, P626

[5] ROLE OF CALCIUM AND PROTEIN-KINASE-C IN THE ACTIVATION OF PHOSPHOLIPASE-D BY ANGIOTENSIN-II IN VASCULAR SMOOTH-MUSCLE CELLS [J].

FREEMAN, EJ ;

CHISOLM, GM ;

TALLANT, EA .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 319 (01) :84-92

[6]

Fulton D, 1996, J PHARMACOL EXP THER, V278, P518

[7] Effects of U73122 and U73343 on human platelet calcium signalling and protein tyrosine phosphorylation [J].

Heemskerk, JWM ;

Farndale, RW ;

Sage, SO .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1997, 1355 (01) :81-88

[8] Human platelet activation is inhibited upstream of the activation of phospholipase A(2) by U73343 [J].

Heemskerk, JWM ;

Feijge, MAH ;

Sage, SO ;

Farndale, RW .

BIOCHEMICAL PHARMACOLOGY, 1997, 53 (09) :1257-1262

[9] HOW IS THE LEVEL OF FREE ARACHIDONIC-ACID CONTROLLED IN MAMMALIAN-CELLS [J].

IRVINE, RF .

BIOCHEMICAL JOURNAL, 1982, 204 (01) :3-16

[10] U73122 INHIBITS PHOSPHOLIPASE C-DEPENDENT CALCIUM MOBILIZATION IN NEURONAL CELLS [J].

JIN, WZ ;

LO, TM ;

LOH, HH ;

THAYER, SA .

BRAIN RESEARCH, 1994, 642 (1-2) :237-243

← 1 2 3 4 →