Histone methyltransferase PRDM8 regulates mouse testis steroidogenesis

被引：69

作者：

Eom, Gwang Hyeon ^{[1
,2
]}

Kim, Kabsun ^{[1
,2
]}

Kim, Sung-Mi ^{[3
]}

Kee, Hae Jin ^{[1
,2
]}

Kim, Ji-Young ^{[3
]}

Jin, Hye Mi ^{[1
,2
]}

Kim, Ju-Ryoung ^{[1
,2
]}

Kim, Jung Ha ^{[1
,2
]}

Choe, Nakwon ^{[1
,2
]}

Kim, Kee-Beom ^{[3
]}

Lee, Junwon ^{[1
,2
]}

Kook, Hyun ^{[1
,2
]}

Kim, Nacksung ^{[1
,2
]}

Seo, Sang-Beom ^{[3
]}

机构：

[1] Chonnam Natl Univ, Sch Med, Med Res Ctr Gene Regulat, Kwangju 501746, South Korea

[2] Chonnam Natl Univ, Sch Med, Dept Pharmacol, Kwangju 501746, South Korea

[3] Chung Ang Univ, Coll Nat Sci, Dept Life Sci, Seoul 156756, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 388卷 / 01期

关键词：

Histone; Methyltransferase; PRDM8; Steroidogenesis; Transcription; TRANSCRIPTIONAL REPRESSION; CARDIAC-HYPERTROPHY; GENE-EXPRESSION; CELLS; DIFFERENTIATION; HOMEODOMAIN; DEACETYLASE;

D O I：

10.1016/j.bbrc.2009.07.134

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A family of PRDM proteins are similar to histone methyltransferases (HMTases) with SET domain in that they modulate different cellular processes, including transcriptional regulation, through chromatin modifying activities. By applying a bioinformatic approach, we searched for proteins containing the SET domain and identified a double zinc-finger domain containing PRDM8 with HMTase activity. In vitro HMTase assay and immunoblot analysis revealed that PRDM8 specifically methylates H3K9 of histones which indicates transcriptional repression activity of PRDM8. Direct recruitment of PRDM8 to the promoter mediated transcriptional repression and indicated no involvement of HDAC. Tissue blot analyses identified PRDM8 transcripts from brain and testis in adult mouse. Consistent with these observations, we demonstrate that PRDM8 repressed the expression of steroidogenic markers, p450c17c and LHR, which indicates its regulatory role in mouse testis development. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：131 / 136

页数：6

共 15 条

[11] Cardiac hypertrophy and histone deacetylase-dependent transcriptional repression mediated by the atypical homeodomain protein Hop [J].