Fluorescent Structural DNA Nanoballs Functionalized with Phosphate-Linked Nucleotide Triphosphates

被引：7

作者：

Anderson, Jon P. ^{[1
]}

Reynolds, Bambi L. ^{[1
]}

Baum, Kristin ^{[2
]}

Williams, John G. ^{[1
]}

机构：

[1] LI COR Biosci Inc, Lincoln, NE 68504 USA

[2] Iowa Dept Publ Safety, Criminalist Lab, Ankeny, IA 50023 USA

来源：

NANO LETTERS | 2010年 / 10卷 / 03期

关键词：

Single molecule sequencing; phosphate-labeled nucleotide; DNA nanoball; SYBR; fluorescent; ARRAYS;

D O I：

10.1021/nl9039718

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Highly labeled DNA nanoballs functionalized with phosphate-linked nucleotide triphosphates (dNTPs) were developed as a source of dNTPs for DNA polymerase The particles were prepared by strand-displacement polymerization from a self-complementary circular template. Imaged by atomic force microscopy, these functionalized particles appear as condensed fuzzy balls with diameters between 50 and 150 nm. They emit a bright fluorescent signal, detected in 2 MS exposures with a signal-to-noise ratio of 25 when imaged using a TIR fluorescence microscope.

引用

页码：788 / 792

页数：5

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