A peroxisomal lon protease and peroxisome degradation by autophagy play key roles in vitality of Hansenula polymorpha cells

被引:77
作者
Aksam, Eda Bener [1 ]
Koek, Anne [1 ]
Kiel, Jan A. K. W. [1 ]
Jourdan, Stefanie [1 ]
Veenhuis, Marten [1 ]
van der Klei, Ida J. [1 ]
机构
[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, NL-9750 AA Haren, Netherlands
关键词
peroxisome; autophagy; lon protease; yeast; housekeeping; ALCOHOL OXIDASE; DIHYDROXYACETONE SYNTHASE; MOLECULAR CHAPERONES; MEMBRANE-PROTEINS; METHANOL OXIDASE; QUALITY-CONTROL; PIM1; PROTEASE; AAA PROTEASES; MITOCHONDRIA; GENE;
D O I
10.4161/auto.3534
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In eukaryote cells various mechanisms exist that are responsible for the removal of non-functional proteins. Here we show that in the yeast Hansenula polymorpha (H. polymorpha) a peroxisomal Lon protease, Pln, plays a role in degradation of unfolded and non-assembled peroxisomal matrix proteins. In addition, we demonstrate that whole peroxisomes are constitutively degraded by autophogy during normal vegetative growth of WT cells. Deletion of both H. polymorpha PLN and ATG 1, required for autophagy, resulted in a significant increase in peroxisome numbers, paralleled by a decrease in cell viability relative to WT cells. Also, in these cells and in cells of PLN and ATG 1 single deletion strains, the intracellular levels of reactive oxygen species had increased relative to WT controls. The enhanced generation of reactive oxygen species may be related to an uneven distribution of peroxisomal catalase activities in the mutant cells, as demonstrated by cytochemistry. We speculate that in the absence of HpPln or autophogy unfolded and non-assembled peroxisomal matrix proteins accumulate, which can form aggregates and lead to an imbalance in hydrogen peroxide production and degradation in some of the organelles.
引用
收藏
页码:96 / 105
页数:10
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