Adhesion of Escherichia coli, Citrobacter freundii and Klebsiella pneumoniae isolated from milk:: consequence on the efficiency of sanitation procedures

The effects of adhesion and of biofilm development on the efficiency of cleaning and disinfection procedures were investigated on three strains belonging to the E coli, C. freundii and K pneumoniae species, which were able to raise more or less complex biofilms. Resistance to a rinsing procedure was strain dependent but not related to the biofilm structure: E coli was poorly adherent although embedded in an organic matrix. Conversely, a similar increase in the heat- and disinfectant-resistance was observed, regardless of the complexity of the biofilm (more or less significant organic matrix). These results suggested the essential role of the bacterial physiological state in resistance to sanitation procedures.