Anti-Cancer Effects of Probiotic Lactobacillus acidophilus for Colorectal Cancer Cell Line Caco-2 through Apoptosis Induction

被引：33

作者：

Isazadeh, Alireza ^{[1
,2
]}

Hajazimian, Saba ^{[1
]}

Shadman, Behrouz ^{[3
]}

Safaei, Sahar ^{[1
]}

Bedoustani, Ahmad Babazadeh ^{[4
]}

Chavoshi, Reza ^{[2
]}

Shanehbandi, Dariush ^{[1
]}

Mashayekhi, Mohammadreza ^{[2
]}

Nahaei, Mohammadreza ^{[5
]}

Baradaran, Behzad ^{[1
,6
]}

机构：

[1] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran

[2] Islamic Azad Univ, Tabriz Branch, Dept Genet, Tabriz, Iran

[3] Ege Univ, Dept Med Biol, Fac Med, Izmir, Turkey

[4] Islamic Azad Univ, Tabriz Branch, Biotechnol Res Ctr, Tabriz, Iran

[5] Islamic Azad Univ, Tabriz Branch, Sch Med, Dept Microbiol & Lab Sci, Tabriz, Iran

[6] Tabriz Univ Med Sci, Dept Immunol, Fac Med, Tabriz, Iran

来源：

PHARMACEUTICAL SCIENCES | 2021年 / 27卷 / 02期

关键词：

Apoptosis; Colorectal cancer; Lactobacillus; Probiotics; LACTIS; CASEI;

D O I：

10.34172/PS.2020.52

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Background: Colorectal cancer is one of the most common cancers worldwide. Probiotics are useful and non-pathogenic microorganisms in the gastrointestinal tract, which can show anticancer activity through the induction of apoptosis. This study aimed to evaluate the anti-proliferative effects of Lactobacillus acidophilus probiotic on the Caco-2 colorectal cancer cell line. Methods: The supernatant (secreted metabolites) and bacterial extract of L. acidophilus probiotics were prepared and used as an anti-proliferative agent on the colorectal cancer cell line, Caco-2 in vitro. The effects of supernatant and extract of L. acidophilus were evaluated on the viability and proliferation of cancer cells using MTT assay. Moreover, morphological alterations of cancer cells treated with supernatant and extract of L. acidophilus were evaluated by an inverted phasecontrast microscope. The mRNA expression levels of apoptosis-related genes (SURVIVIN and SMAC) in treated cancer cells and untreated controls were evaluated using the Real-Time PCR method. Results: The results showed that the supernatant and extract of L. acidophilus inhibited the viability and proliferation of cancer cells in a dose and time-dependent manner. Moreover, various morphological alterations were observed in the treated cancer cells, which are indicators of apoptosis induction. The mRNA expression of SURVIVIN and SMAC genes were significantly up-regulated and downregulated in the treated cancer cells, respectively. Conclusion: The results of the present study suggested that the supernatant and extract of L. acidophilus could inhibit the viability and proliferation of colorectal cancer cell line, Caco-2 through induction of apoptosis.increase the survival rate of colon cancer patients.

引用

页码：262 / 267

页数：6

共 24 条

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