In vivo visualization of the distribution of a secretory protein in Aspergillus oryzae hyphae using the RntA-EGFP fusion protein

被引：30
作者：
Masai, K
Maruyama, J
Nakajima, H
Kitamoto, K
机构：
[1] Univ Tokyo, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan
[2] BRAIN, Biooriented Technol Res Adv Institut, Omiya, Saitama 3318537, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2003年 / 67卷 / 02期
关键词：
Aspergillus oryzae; secretory pathway; fusion protein; EGFP;
D O I：
10.1271/bbb.67.455
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A fusion gene encoding ribonuclease T-1-EGFP (rntA-egfp) was constructed and expressed to use it as a tool for studies on the secretory pathway in Aspergillus oryzae. The successful secretion of the intact RntA-EGFP fusion protein was detected by fluorescence measurement and Western analysis. With use of the RntA-EGFP system, we were able to see high fluorescence at hyphal tips and observe concentrated fluorescence at septa in basal cells during growth at optimal conditions. Cold or heat shock during growth caused the accumulation of EGFP fluorescence in vacuoles.
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页码：455 / 459
页数：5
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