Styrene maleic acid copolymer induces pores in biomembranes

被引：15

作者：

Rydmark, Marcella Orwick ^{[1
]}

Christensen, Mikkel Killingmoe ^{[2
]}

Koksal, Elif Senem ^{[2
]}

Kantarci, Ilayda ^{[2
]}

Kustanovich, Kiryl ^{[3
]}

Yantchev, Ventsislav ^{[3
]}

Jesorka, Aldo ^{[3
]}

Gozen, Irep ^{[2
,3
,4
]}

机构：

[1] Univ Oslo, Dept Biosci, N-0316 Oslo, Norway

[2] Univ Oslo, Fac Med, Ctr Mol Med Norway, N-0318 Oslo, Norway

[3] Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden

[4] Univ Oslo, Fac Math & Nat Sci, N-0315 Oslo, Norway

来源：

SOFT MATTER | 2019年 / 15卷 / 39期

基金：

欧盟地平线“2020”; 瑞典研究理事会;

关键词：

MEMBRANE-PROTEINS; PARTICLES; MIXTURES; VESICLES; INTACT; TOOL;

D O I：

10.1039/c9sm01407a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We investigated the interactions between styrene-maleic acid (SMA) copolymers and phospholipid bilayers, using confocal microscopy and surface acoustic wave resonance (SAR) sensing. For the first time we experimentally observed and followed pore formation by SMA copolymers in intact supported bilayers and unilamellar vesicles, showing that fluorescein, a water-soluble organic compound with a mean diameter of 6.9 angstrom, can traverse the membrane. Our findings are in agreement with recent theoretical predictions, which suggested that SMA copolymers may insert along the plane of the bilayer to form stable toroidal pores.

引用

页码：7934 / 7944

页数：11

共 50 条

[1] The Styrene-Maleic Acid Copolymer Extracts Active Complexes from Native Biomembranes
Long, Ashley R.
O'Brien, Catherine C.
Malhotra, Ketan
Albert, Arlene D.
Watts, Anthony
Alder, Nathan N.
BIOPHYSICAL JOURNAL, 2013, 104 (02) : 523A - 523A
[2] POTENTIOMETRIC TITRATIONS OF A POLYDICARBOXYLIC ACID - MALEIC ACID STYRENE COPOLYMER
GARRETT, ER
GUILE, RL
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1951, 73 (10) : 4533 - 4535
[3] HYDROPHOBIC INTERACTION IN MONOETHERS OF STYRENE COPOLYMER AND MALEIC ACID
BOIKO, VP
UKRAINSKII KHIMICHESKII ZHURNAL, 1976, 42 (02): : 208 - 210
[4] Adsorption of the copolymer of styrene with the sodium salts of maleic acid by aerosil
Tsapko, M.D.
Baran, A.A.
Mitina, N.S.
Bratunets, A.G.
Kolloidnyi Zhurnal, 1991, 53 (05): : 934 - 939
[5] Copolymer from the waste products of styrene and maleic acid production
Filimonova, O. N.
RUSSIAN JOURNAL OF APPLIED CHEMISTRY, 2007, 80 (07) : 1213 - 1217
[6] Copolymer from the waste products of styrene and maleic acid production
O. N. Filimonova
Russian Journal of Applied Chemistry, 2007, 80 : 1213 - 1217
[7] The styrene–maleic acid copolymer: a versatile tool in membrane research
Jonas M. Dörr
Stefan Scheidelaar
Martijn C. Koorengevel
Juan J. Dominguez
Marre Schäfer
Cornelis A. van Walree
J. Antoinette Killian
European Biophysics Journal, 2016, 45 : 3 - 21
[8] Solubilization of lipids and lipid phases by the styrene–maleic acid copolymer
Juan J. Dominguez Pardo
Jonas M. Dörr
Aditya Iyer
Ruud C. Cox
Stefan Scheidelaar
Martijn C. Koorengevel
Vinod Subramaniam
J. Antoinette Killian
European Biophysics Journal, 2017, 46 : 91 - 101
[9] INTERACTION OF STYRENE MALEIC-ANHYDRIDE COPOLYMER WITH STYRENE
BARTON, J
VASKOVA, V
MAKROMOLEKULARE CHEMIE-MACROMOLECULAR CHEMISTRY AND PHYSICS, 1980, 181 (01): : 261 - 265
[10] Solubilization of lipids and lipid phases by the styrene-maleic acid copolymer
Pardo, Juan J. Dominguez
Dorr, Jonas M.
Iyer, Aditya
Cox, Ruud C.
Scheidelaar, Stefan
Koorengevel, Martijn C.
Subramaniam, Vinod
Killian, J. Antoinette
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2017, 46 (01): : 91 - 101

← 1 2 3 4 5 →