Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case

被引：6

作者：

Feng, Lei ^{[1
,2
]}

Xu, Cheng ^{[3
]}

Zeng, Xianhai ^{[4
]}

Zhang, Huaicai ^{[5
]}

Yang, Fan ^{[3
]}

Li, Wanshui ^{[1
,2
]}

Tu, Zheng ^{[1
,2
]}

Li, Caixia ^{[1
,2
]}

Hu, Lan ^{[1
,2
]}

机构：

[1] Minist Publ Secur, Inst Forens Sci, Beijing 100038, Peoples R China

[2] Minist Publ Secur, Key Lab Forens Genet, Beijing, Peoples R China

[3] Chinese Peoples Publ Secur Univ, Beijing, Peoples R China

[4] Publ Secur Bur Fujian Prov, Forens Technol Div, Fuzhou, Peoples R China

[5] Publ Secur Bur Hangzhou City, Inst Criminal Sci & Technol, Hangzhou, Zhejiang, Peoples R China

来源：

CROATIAN MEDICAL JOURNAL | 2014年 / 55卷 / 05期

关键词：

LASER MICRODISSECTION; DNA; STRS;

D O I：

10.3325/cmj.2014.55.537

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Aim To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler (R) kit. Consensus DNA profiles were generated from 5 replicate experiments. Results Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained. Conclusion The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.

引用

页码：537 / 541

页数：5

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