Improving poly-(γ-glutamic acid) production from a glutamic acid-independent strain from inulin substrate by consolidated bioprocessing

被引:19
作者
Qiu, Yibin [1 ,2 ]
Zhang, Yatao [1 ,2 ]
Zhu, Yifan [1 ,2 ]
Sha, Yuanyuan [1 ,2 ]
Xu, Zongqi [1 ,2 ]
Feng, Xiaohai [1 ,2 ]
Li, Sha [1 ,2 ]
Xu, Hong [1 ,2 ]
机构
[1] State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Jiangsu, Peoples R China
[2] Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Jiangsu, Peoples R China
关键词
gamma-PGA; Glutamic acid-independent; Inulin extract; Saccharification strategy; Immobilized inulinase; JERUSALEM-ARTICHOKE TUBERS; POLY-GAMMA-GLUTAMATE; POLY(GAMMA-GLUTAMIC ACID); ETHANOL-PRODUCTION; CANE MOLASSES; OPTIMIZATION; FERMENTATION; PLASMA; GENE; L;
D O I
10.1007/s00449-019-02167-w
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To excavate the application of Jerusalem artichoke on poly(gamma-glutamic acid) (gamma-PGA) production, a gamma-PGA producing strain Bacillus amyloliquefaciens NX-2S154 was obtained through atmospheric and room temperature plasma mutagenesis, which produced 14.83 +/- 0.31 g/L of gamma-PGA in batch fermentation with raw inulin extract. Simultaneous saccharification and fermentation (SSF) by adding commercial inulinase were further investigated for gamma-PGA fermentation. Results showed SSF could eliminate the ineffective utilization of inulin while avoiding inhibition effect of high concentration substrate, which made gamma-PGA concentration reach 18.54 +/- 0.39 g/L with the process being shortened by 17%. Finally, an immobilized column for reducing inulinase cost was introduced to gamma-PGA production. Repeated batch cultures showed the novel bioreactor exhibited higher stability and simplicity and gave average gamma-PGA concentration and productivity of 19.40 +/- 0.37 g/L and 0.27 +/- 0.008 g/L/h, respectively. This work proposes a productive method for efficient gamma-PGA production using Jerusalem artichoke feedstock.
引用
收藏
页码:1711 / 1720
页数:10
相关论文
共 41 条
[1]   Isolation of Bacillus subtilis (chungkookjang), a poly-γ-glutamate producer with high genetic competence [J].
Ashiuchi, M ;
Kamei, T ;
Baek, DH ;
Shin, SY ;
Sung, MH ;
Soda, K ;
Yagi, T ;
Misono, H .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2001, 57 (5-6) :764-769
[2]   Biochemistry and molecular genetics of poly-γ-glutamate synthesis [J].
Ashiuchi, M ;
Misono, H .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 59 (01) :9-14
[3]   A statistical approach to optimization of fermentative production of poly(γ-glutamic acid) from Bacillus licheniformis NCIM 2324 [J].
Bajaj, I. B. ;
Lele, S. S. ;
Singhal, R. S. .
BIORESOURCE TECHNOLOGY, 2009, 100 (02) :826-832
[4]   Glutamic acid independent production of poly-γ-glutamic acid by Bacillus amyloliquefaciens LL3 and cloning of pgsBCA genes [J].
Cao, Mingfeng ;
Geng, Weitao ;
Liu, Li ;
Song, Cunjiang ;
Xie, Hui ;
GuoA, Wenbin ;
Jin, Yinghong ;
Wang, Shufang .
BIORESOURCE TECHNOLOGY, 2011, 102 (05) :4251-4257
[5]   Stability and catalytic kinetics of acid phosphatase immobilized on composite beads of chitosan and activated clay [J].
Chang, MY ;
Juang, RS .
PROCESS BIOCHEMISTRY, 2004, 39 (09) :1087-1091
[6]   Inulinase-expressing microorganisms and applications of inulinases [J].
Chi, Zhenming ;
Chi, Zhe ;
Zhang, Tong ;
Liu, Guanglei ;
Yue, Lixi .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 82 (02) :211-220
[7]   Photothermal-modulated drug delivery and magnetic relaxation based on collagen/poly(γ-glutamic acid) hydrogel [J].
Cho, Sun-Hee ;
Kim, Ahreum ;
Shin, Woojung ;
Heo, Min Beom ;
Noh, Hyun Jong ;
Hong, Kwan Soo ;
Cho, Jee-Hyun ;
Lim, Yong Taik .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2017, 12 :2607-2620
[8]   Characterization of inulin hydrolyzing enzyme(s) in commercial glucoamylases and its application in lactic acid production from Jerusalem artichoke tubers (Jat) [J].
Dao, Thai Ha ;
Zhang, Jian ;
Bao, Jie .
BIORESOURCE TECHNOLOGY, 2013, 148 :157-162
[9]   Significantly enhanced substrate tolerance of Pseudomonas putida nitrilase via atmospheric and room temperature plasma and cell immobilization [J].
Dong, Ting-Ting ;
Gong, Jin-Song ;
Gu, Bing-Chen ;
Zhang, Qiang ;
Li, Heng ;
Lu, Zhen-Ming ;
Lu, Mao-Lin ;
Shi, Jin-Song ;
Xu, Zheng-Hong .
BIORESOURCE TECHNOLOGY, 2017, 244 :1104-1110
[10]   Efficient production of poly--glutamic acid from cane molasses by Bacillus subtilis NX-2 immobilized on chemically modified sugarcane bagasse [J].
Feng, Xiaohai ;
Tang, Bao ;
Jiang, Yongxiang ;
Xu, Zongqi ;
Lei, Peng ;
Liang, Jinfeng ;
Xu, Hong .
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2016, 91 (07) :2085-2093