Extracellular overproduction and preliminary crystallographic analysis of a family I.3 lipase

被引：8

作者：

Angkawidjaja, Clement

You, Dong-Ju

Matsumura, Hiroyoshi

Koga, Yuichi

Takano, Kazufumi

Kanaya, Shigenori

机构：

[1] Osaka Univ, Dept Mat & Life Sci, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Dept Appl Chem, Grad Sch Engn, Suita, Osaka 5650871, Japan

[3] CREST, Sosho Project, JST, Suita, Osaka 5650871, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2007年 / 63卷

关键词：

D O I：

10.1107/S1744309107004575

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A family I. 3 lipase from Pseudomonas sp. MIS38 was secreted from Escherichia coli cells to the external medium, purified and crystallized and preliminary crystallographic studies were performed. The crystal was grown at 277 K by the hanging-drop vapour-diffusion method. Native X-ray diffraction data were collected to 1.7 angstrom resolution using synchrotron radiation at station BL38B1, SPring-8. The crystal belongs to space group P2(1), with unit-cell parameters a = 48.79, b = 84.06, c = 87.04 angstrom. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient V-M was calculated to be 2.73 angstrom(3) Da(-1) and the solvent content was 55%.

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页码：187 / 189

页数：3

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