Nutritional requirements of Lactobacillus delbrueckii subsp lactis in a chemically defined medium

被引：75

作者：

Hébert, EM

Raya, RR

de Giori, GS

机构：

[1] Consejo Nacl Invest Cient & Tecn, Ctr Referencia Lactobacilos CERELA, RA-4000 San Miguel De Tucuman, Argentina

[2] Univ Nacl Tucuman, Fac Bioquim Quim & Farm, Catedra Microbiol Super, RA-4000 San Miguel De Tucuman, Tucuman, Argentina

来源：

CURRENT MICROBIOLOGY | 2004年 / 49卷 / 05期

关键词：

D O I：

10.1007/s00284-004-4357-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study was undertaken to determine the nutritional requirements of Lactobacillus delbrueckii subsp. lactis and to develop a minimal chemically defined medium that supports sustained growth of these microorganisms. The single-omission technique was applied to each component of complete chemically defined medium in order to determine the nutritional requirements. L. delbrueckii subsp. lactis was prototrophic for alanine, glycine, aspartic acid, asparagine, glutamine, threonine, and proline. The lysine requirement was strain-dependent. Magnesium was the only essential oligoelement. These microorganisms also required uracil and guanine and adenine as pyrimidine and purine sources, respectively. In view of the nutritional requirements we designed a new minimal defined medium which supports sustained growth of L. delbrueckii subsp. lactis. This medium is simple and well defined, and should be preferable to complex media for conducting future biochemical, physiological, and genetic studies on L. delbrueckii subsp. lactis.

引用

页码：341 / 345

页数：5

共 19 条

[1] Extent of genetic lesions of the arginine and pyrimidine biosynthetic pathways in Lactobacillus plantarum, L-paraplantarum, L-pentosus, and L-casei:: Prevalence of CO2-dependent auxotrophs and characterization of deficient arg genes in L-plantarum [J].

Bringel, F ;

Hubert, JC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (05) :2674-2683

[2] Physiological study of Lactobacillus delbrueckii subsp bulgaricus strains in a novel chemically defined medium [J].

Chervaux, C ;

Ehrlich, SD ;

Maguin, E .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (12) :5306-5311

[3] RATIONAL DEVELOPMENT OF A SIMPLE SYNTHETIC MEDIUM FOR THE SUSTAINED GROWTH OF LACTOCOCCUS-LACTIS [J].

COCAIGNBOUSQUET, M ;

GARRIGUES, C ;

NOVAK, L ;

LINDLEY, ND ;

LOUBIERE, P .

JOURNAL OF APPLIED BACTERIOLOGY, 1995, 79 (01) :108-116

[4]

DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x

[5] Iron requirement of Lactobacillus spp. in completely chemically defined growth media [J].

Elli, M ;

Zink, R ;

Rytz, A ;

Reniero, R ;

Morelli, L .

JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (04) :695-703

[6] Cardiovascular effects of fermented milk containing angiotensin-converting enzyme inhibitors evaluated in permanently catheterized, spontaneously hypertensive rats [J].

Fuglsang, A ;

Nilsson, D ;

Nyborg, NCB .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (07) :3566-3569

[7]

Grobben GJ, 1998, APPL ENVIRON MICROB, V64, P1333

[8] Modulation of the cell-surface proteinase activity of thermophilic lactobacilli by the peptide supply [J].

Hébert, EM ;

Raya, RR ;

de Giori, GS .

CURRENT MICROBIOLOGY, 2002, 45 (06) :385-389

[9] Nutritional requirements and nitrogen-dependent regulation of proteinase activity of Lactobacillus helveticus CRL 1062 [J].

Hebert, EM ;

Raya, RR ;

De Giori, GS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (12) :5316-5321

[10] CARBOHYDRATE-METABOLISM IN LACTIC-ACID BACTERIA [J].

KANDLER, O .

ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY, 1983, 49 (03) :209-224

← 1 2 →