Biolistic® transformation of cacao [Theobroma cacao (L.)]

被引：0
|
作者：
Perry, MD [1 ]
Power, JB [1 ]
Lowe, KC [1 ]
Davey, MR [1 ]
机构：
[1] Univ Nottingham, Sch Biol Sci, Plant Sci Div, Nottingham NG7 2RD, England
来源：
TROPICAL AGRICULTURE | 2000年 / 77卷 / 01期
关键词：
microprojectile bombardment; woody plant; Theobroma cacao;
D O I：
暂无
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Callus of cacao [Theobroma cacao (L.)] were transformed by particle bombardment (Biolistics(R)). Four-week-old floral-derived calli were bombarded with plasmid containing the beta -glucuronidase (gus) gene, driven by a CaMV 35S promoter. Histochemical analysis of gus gene expression was used to optimize the parameters for deoxyribonucleic acid (DNA) delivery. More than 270 histochemically positive (blue staining) transformation events were observed with staminode-derived calli bombarded at 1550 psi.
引用
收藏
页码：64 / 66
页数：3
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