Optimal analytical conditions for catalase in fresh water prawn, Macrobrachium malcolmsonii

被引：9

作者：

Arun, S ^{[1
]}

Thirumurugan, R ^{[1
]}

Visakan, R ^{[1
]}

Balamurugan, S ^{[1
]}

Arunachalam, V ^{[1
]}

Subramanian, P ^{[1
]}

机构：

[1] Bharathidasan Univ, Dept Anim Sci, Tiruchirappalli 620024, India

来源：

BIOTECHNIC & HISTOCHEMISTRY | 2003年 / 78卷 / 01期

关键词：

Arrhenius plot; catalase; energy activation; energy inhibition; Lineweaver-Burk plot; M; malcolmsonii;

D O I：

10.1080/10520290312120001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The cytosol of hepatopancreas was prepared from the freshwater prawn Macrobrachium malcolmsonii, and optimal assay conditions, i.e., concentration of substrate, pH, and temperature, were determined to measure basal activities and kinetic constants of catalase activity. The properties of catalase were examined in M. macolmsonii, because quantitative data on catalase are limited in crustacean species. The optimal pH for catalase was 7.0. The activation energy was 3.55 Kcal/mol and energy inhibition value was 5.16 Kcal/mol. The value of energy inhibition is higher than that of energy activation. This may be due to inhibition of catalase by some substrate other than H2O2. A Km of 66.6 mM was also determined from various concentrations of substrate.

引用

页码：1 / 4

页数：4

共 17 条

[1]

ABEI H, 1984, METHOD ENZYMAT AN, V2, P673

[2] Antioxidant enzymes activity in subcellular fraction of freshwater prawns M-malcolmsonii and M-lamarrei lamarrei [J].

Arun, S ;

Subramanian, P .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1998, 75 (2-3) :187-192

[3]

Arun S, 1997, CURR SCI INDIA, V73, P107

[4] Properties of glutathione peroxidase from the hepatopancreas of freshwater prawn Macrobrachium malcolmsonii [J].

Arun, S ;

Krishnamoorthy, P ;

Subramanian, P .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 1999, 31 (06) :725-732

[5] Antioxidant enzymes in freshwater prawn Macrobrachium malcolmsonii during embryonic and larval development [J].

Arun, S ;

Subramanian, P .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1998, 121 (03) :273-277

[6]

ARUN S, 2000, THESIS BHARATHIDASAN

[7]

BORG DC, 1984, ISR J CHEM, V24, P38

[8] BIOLOGICAL ROLE OF SUPEROXIDE ION-RADICAL [J].

BYCZKOWSKI, JZ ;

GESSNER, T .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1988, 20 (06) :569-580

[9]

Claiborne A., 1985, Handbook of Methods of Oxygen Radicals Research, DOI DOI 10.1016/0531-5565(85)90021-X

[10]

Davies KJA, 1995, BIOCHEM SOC SYMP, P1, DOI 10.1042/bss0610001

← 1 2 →