Optimal analytical conditions for catalase in fresh water prawn, Macrobrachium malcolmsonii

被引:9
作者
Arun, S [1 ]
Thirumurugan, R [1 ]
Visakan, R [1 ]
Balamurugan, S [1 ]
Arunachalam, V [1 ]
Subramanian, P [1 ]
机构
[1] Bharathidasan Univ, Dept Anim Sci, Tiruchirappalli 620024, India
关键词
Arrhenius plot; catalase; energy activation; energy inhibition; Lineweaver-Burk plot; M; malcolmsonii;
D O I
10.1080/10520290312120001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The cytosol of hepatopancreas was prepared from the freshwater prawn Macrobrachium malcolmsonii, and optimal assay conditions, i.e., concentration of substrate, pH, and temperature, were determined to measure basal activities and kinetic constants of catalase activity. The properties of catalase were examined in M. macolmsonii, because quantitative data on catalase are limited in crustacean species. The optimal pH for catalase was 7.0. The activation energy was 3.55 Kcal/mol and energy inhibition value was 5.16 Kcal/mol. The value of energy inhibition is higher than that of energy activation. This may be due to inhibition of catalase by some substrate other than H2O2. A Km of 66.6 mM was also determined from various concentrations of substrate.
引用
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页码:1 / 4
页数:4
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