Long non-coding RNA ATB promotes malignancy of esophageal squamous cell carcinoma by regulating miR-200b/Kindlin-2 axis

被引：74
作者：
Li, Zhongwen [1 ]
Wu, Xiaoliang [2 ,3 ,4 ]
Gu, Ling [3 ,4 ,5 ]
Shen, Qi [2 ]
Luo, Wen [2 ]
Deng, Chuangzhong [3 ,4 ]
Zhou, Qianghua [3 ,4 ]
Chen, Xinru [3 ,4 ]
Li, Yanjie [6 ]
Lim, ZuanFu [7 ]
Wang, Xing [3 ,4 ]
Wang, Jiahong [3 ,4 ,8 ]
Yang, Xianzi [3 ,4 ,8 ]
机构：
[1] Zunyi Med Univ, Dept Oncol, Affiliated Hosp, Zunyi, Peoples R China
[2] Guizhou Prov Peoples Hosp, Dept Oncol, Guiyang, Guizhou, Peoples R China
[3] Sun Yat Sen Univ, Canc Ctr, State Key Lab Oncol South China, Guangzhou, Guangdong, Peoples R China
[4] Sun Yat Sen Univ, Canc Ctr, Collaborat Innovat Ctr Canc Med, Guangzhou, Guangdong, Peoples R China
[5] Puer Univ, Puer, Peoples R China
[6] Sun Yat Sen Univ, Affiliated Hosp 3, Guangzhou, Guangdong, Peoples R China
[7] West Virginia Univ, Mary Babb Randolph Canc Inst, WVU Canc Inst, Robert C Byrd Hlth Sci Ctr, Morgantown, WV USA
[8] Guangzhou Med Univ, Affiliated Canc Hosp & Inst, 78 Hengzhigang Rd, Guangzhou, Guangdong, Peoples R China
来源：
CELL DEATH & DISEASE | 2017年 / 8卷
关键词：
INVASION-METASTASIS CASCADE; LNCRNA-ATB; UP-REGULATION; CANCER CELLS; KINDLIN-2; PROGRESSION; INVASIVENESS; PROGNOSIS; EVOLUTION; MIGRATION;
D O I：
10.1038/cddis.2017.245
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer-related death, especially in China. In addition, the prognosis of late stage patients is extremely poor. However, the biological significance of the long non-coding RNA lnc-ATB and its potential role in ESCC remain to be documented. In this study, we investigated the role of lnc-ATB and the underlying mechanism promoting its oncogenic activity in ESCC. Expression of lnc-ATB was higher in ESCC tissues and cell lines than that in normal counterparts. Upregulated lnc-ATB served as an independent prognosis predictor of ESCC patients. Moreover, loss-offunction assays in ESCC cells showed that knockdown of lnc-ATB inhibited cell proliferation and migration both in vitro and in vivo. Mechanistic investigation indicated that lnc-ATB exerted oncogenic activities via regulating Kindlin-2, as the anti-migration role of lnc-ATB silence was attenuated by ectopic expression of Kindlin-2. Further analysis showed that lnc-ATB functions as a molecular sponge for miR-200b and Kindlin-2. Dysregulated miR-200b/Kindlin-2 signaling mediated the oncogenic activity of lnc-ATB in ESCC. Our results suggest that lnc-ATB predicts poor prognosis and may serve as a potential therapeutic target for ESCC patients.
引用
收藏
页码：e2888 / e2888
页数：11
相关论文
共 37 条
[1]  
Adenis A, 2015, NEW ENGL J MED, V372, P1471, DOI 10.1056/NEJMc1500692
[2]   Kindlin-2 is expressed in malignant mesothelioma and is required for tumor cell adhesion and migration [J].
An, Zhengwen ;
Dobra, Katalin ;
Lock, John G. ;
Stromblad, Staffan ;
Hjerpe, Anders ;
Zhang, Hongquan .
INTERNATIONAL JOURNAL OF CANCER, 2010, 127 (09) :1999-2008
[3]   EMT: 2016 [J].
Angela Nieto, M. ;
Huang, Ruby Yun-Ju ;
Jackson, Rebecca A. ;
Thiery, Jean Paul .
CELL, 2016, 166 (01) :21-45
[4]  
[Anonymous], 2018, ANTI-CANCER DRUG, DOI [DOI 10.3322/caac.20115, DOI 10.1097/CAD.0000000000000617]
[5]   Dysregulation of Wnt-Signaling and a Candidate Set of miRNAs Underlie the Effect of Metformin on Neural Crest Cell Development [J].
Banerjee, Poulomi ;
Dutta, Sunit ;
Pal, Rajarshi .
STEM CELLS, 2016, 34 (02) :334-345
[6]   Long Noncoding RNAs: Cellular Address Codes in Development and Disease [J].
Batista, Pedro J. ;
Chang, Howard Y. .
CELL, 2013, 152 (06) :1298-1307
[7]   Cancer Statistics in China, 2015 [J].
Chen, Wanqing ;
Zheng, Rongshou ;
Baade, Peter D. ;
Zhang, Siwei ;
Zeng, Hongmei ;
Bray, Freddie ;
Jemal, Ahmedin ;
Yu, Xue Qin ;
He, Jie .
CA-A CANCER JOURNAL FOR CLINICIANS, 2016, 66 (02) :115-132
[8]   Upregulation of long noncoding RNA HOTTIP promotes metastasis of esophageal squamous cell carcinoma via induction of EMT [J].
Chen, Xuemei ;
Han, Hongyu ;
Li, Yuqi ;
Zhang, Qiongxia ;
Mo, Kailan ;
Chen, Size .
ONCOTARGET, 2016, 7 (51) :84480-84485
[9]   Long non-coding RNA Loc554202 induces apoptosis in colorectal cancer cells via the caspase cleavage cascades [J].
Ding, Jie ;
Lu, Binbin ;
Wang, Jianping ;
Wang, Juan ;
Shi, Yongguo ;
Lian, Yifan ;
Zhu, Ya ;
Wang, Jirong ;
Fan, Yingrui ;
Wang, Zhaoxia ;
De, Wei ;
Wang, Keming .
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2015, 34
[10]   MiR-200, a new star miRNA in human cancer [J].
Feng, Xiangling ;
Wang, Zhengming ;
Fillmore, Rebecca ;
Xi, Yaguang .
CANCER LETTERS, 2014, 344 (02) :166-173
1234