Induction Mechanism of Heme Oxygenase-1 and Anti-inflammatory Activity by Agaro-oligosaccharides

被引：0

作者：

Enoki, Tatsuji ^{[1
]}

Tanabe, Masashige ^{[1
]}

Shimomura, Mayumi ^{[1
]}

Ohnogi, Hiromu ^{[1
]}

机构：

[1] Takara Bio Inc, Biotechnol Res Labs, Shiga 5202193, Japan

来源：

JOURNAL OF THE JAPANESE SOCIETY FOR FOOD SCIENCE AND TECHNOLOGY-NIPPON SHOKUHIN KAGAKU KOGAKU KAISHI | 2010年 / 57卷 / 04期

关键词：

agar; agaro-oligosaccharide; heme oxygenase-1; anti-inflammation; KAPPA-B; CHEMOPREVENTION; CARCINOGENESIS; EXPRESSION; CANCER; GENE; NRF2;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Agaro-oligosaccharides (AGOs) are easily produced from agar under acidic conditions. We previously reported inhibitory action of AGOs on the production of pro-inflammatory mediators, such as nitric oxide, prostaglandin E-2, pro-inflammatory cytokines, in LPS-stimulated monocyte/macrophages. This inhibitory activity is thought to result from the induction of heme oxygenase-1 (HO-1). Here, we further clarified the induction mechanism of HO-1 and its involvement in the anti-inflammatory activity of AGOs. HO-1 expression is known to be regulated by the transcription factor, nuclear factor E2-related factor-2. We verified that siRNA of this transcription factor could attenuate the induction of HO-1 by AGOs. The production of some pro-inflammatory mediators has been associated with mitogenic stimuli (i.e., LPS), followed by activation of transcription factor, nuclear factor-kappa B. We found that AGOs partially suppressed LPS-mediated nuclear factor-kappa B activation, whereas HO-1 siRNA showed no effect on this inhibitory activity. Taken together, AGOs appear to exert their anti-inflammatory effects on activated macrophages through both HO-1-dependent and -independent mechanisms.

引用

页码：157 / 162

页数：6

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