Electrochemical biosensing of mosquito-borne viral disease, dengue: A review

Dengue virus is a mosquito-borne, single positive-stranded RNA virus that spread human being through infected female Aedes mosquito bite and causes dengue fever. The demand for early detection of this virus has increased to control the widespread of infectious diseases and protect humankind from its harmful effects. Recently, biosensors are found to the potential tool to detect and quantify the virus with fast detection, relatively cost-effective, high sensitivity and selectivity than the conventional diagnostic methods such as immunological and molecular techniques. Mostly, the biosensors employ electrochemical detection technique with transducers, owing to its easy construction, low-cost, ease of use, and portability. Here, we review the current trends and advancement in the electrochemical diagnosis of dengue virus and discussed various types of electrochemical biosensing techniques such as; amperometric, potentiometric, impedometric, and voltammetric sensing. Apart from these, we discussed the role of biorecognition molecules such as nucleic acid, antibodies, and lectins in electrochemical sensing of dengue virus. In addition, the review highlighted the benefits of the electrochemical approach in comparison with traditional diagnostic methods. We expect that these dengue virus diagnostic techniques will continue to evolve and grow in future, with exciting new possibilities stemming from advancement in the rational design of electrochemical biosensors.