Oil/mineral-salts medium designed for easy recovery of extracellular lipase from Fusarium oxysporum AM3

被引：9

作者：

Camargo-de-Morais, MM

Maia, MMD

Borba, FFS

Melo, KG

Santos, CMSO

Reis, ERA

Morais, MA

Lima, JL

机构：

[1] UFPE, LIKA, Setor Biotecnol, BR-50670901 Recife, PE, Brazil

[2] Univ Fed Pernambuco, Dept Genet, Recife, PE, Brazil

[3] Univ Fed Pernambuco, Inst Ciencias Biol, Recife, PE, Brazil

[4] Univ Fed Rural Pernambuco, Dept Biol, Recife, PE, Brazil

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2003年 / 19卷 / 01期

关键词：

enzyme production; factorial analysis; Fusarium; lipase; protein purification;

D O I：

10.1023/A:1022543125420

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipase production by the potato pathogen Fusarium oxysporum AM3 was investigated in a mineral medium using triolein and sodium nitrate as carbon and nitrogen sources, respectively. Medium design by factorial analysis of the medium components increased enzyme activity 9.4-folds over the standard medium. The simple medium composition promoted easy enzyme recovery to its homogeneity in a single step. The lipase showed optimum activity at pH 9.0 and 35 degreesC, with a K-M value of 7.5 mM for triolein and apparent molecular weight of 29.0 kDa. When assayed with different solvents, FoxAM3 lipase showed an increase on its activity by isooctane, isopropanol and acetone.

引用

页码：17 / 20

页数：4

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[2]

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[4]

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