Development of an indirect ELISA for the serological diagnosis of dourine

被引：4

作者：

Bonfini, Barbara ^{[1
]}

Tittarelli, Manuela ^{[1
]}

Luciani, Mirella ^{[1
]}

Di Pancrazio, Chiara ^{[1
]}

Rodomonti, Diamante ^{[1
]}

Iannetti, Luigi ^{[1
]}

Vulpiani, Michele Podaliri ^{[1
]}

Di Febo, Tiziana ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Abruzzo & Molise G, Via Campo Boario, I-64100 Teramo, Italy

来源：

VETERINARY PARASITOLOGY | 2018年 / 261卷

关键词：

Dourine; iELISA; Immunoblotting; Serology; Trypanosoma equiperdum; TRYPANOSOMA-EQUIPERDUM; SERODIAGNOSIS;

D O I：

10.1016/j.vetpar.2018.08.014

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Dourine is a parasitic venereal disease of equines caused by T. equiperdum. Humoral antibodies are found in infected animals, but diagnosis of dourine must include history, clinical, and pathological findings in addition to serology. Complement Fixation Test (CFT) is the Office International des Epizooties (OIE) recommended test for international trade; however, some uninfected equines may give inconsistent or nonspecific reactions in CFT due to the anticomplementary effects of their sera. In this study an Indirect Enzyme Linked Immunosorbent Assay (iELISA) was developed. This test could be used to confirm positive serological cases of dourine or to solve inconclusive results obtained by CFT, in addition to Indirect Fluorescent Antibody Test (IFAT) and a Chemiluminescent Immunoblotting Assay (cIB). Six-hundred-and-six CFT negative sera and 140 sera positive to CFT and IFAT were tested by iELISA using OVI C equiperdum as antigen. Results were expressed as percentage of positivity and the optimum cut-off value determined sensitivity and specificity of 100%. All positive sera, tested by cIB, were confirmed as positive. Additionally, twenty seven sera, low-positive at CFT and negative by IFAT, were tested with iELISA and cIB. All samples resulted negative by clB and one of them was positive in ELISA. Our results suggest that iELISA and cIB may be used as alternative or supplementary confirmatory tests whenever other recommended serological methods are inconclusive or doubtful.

引用

页码：86 / 90

页数：5

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