The effects of seasonal changes on the molecular weight of Nephila clavata spider silk

被引：7

作者：

Osaki, Shigeyoshi ^{[1
]}

Yamamoto, Keizo ^{[2
]}

Matsuhira, Takashi ^{[2
]}

Sakai, Hiromi ^{[2
]}

机构：

[1] Nara Med Univ, Dept Dermatol, 840 Shijo Cho, Kashihara, Nara 6348521, Japan

[2] Nara Med Univ, Dept Chem, Nara, Japan

来源：

POLYMER JOURNAL | 2016年 / 48卷 / 05期

关键词：

CLAVIPES DRAGLINE SILK; MECHANICAL LIFELINE; IRRADIATION; FIBERS; CELLS;

D O I：

10.1038/pj.2015.138

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Silk proteins were taken from the major ampullate glands found in the abdomens of Nephila clavata spiders that were collected on different days during an autumn season in Japan. The molecular weights of the silk proteins taken from 125 spiders were determined under a reduced state using electrophoresis. It was found that the molecular weight of N. clavata spider silk protein changed depending on the time period during the autumn season and showed a peak value of similar to 300 kDa during mating season. However, the molecular weight of N. clavata spider silk protein was, on average, similar to 270 kDa except during mating season. Such a peak in the molecular weight for female N. clavata may be ascribed to the necessity for building mechanically strong orb-webs, which consist of silk with relatively large proteins for the purpose of accepting many male spiders.

引用

页码：659 / 663

页数：5

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