Phenotypic and genotypic characterization of two Toxoplasma gondii isolates in free-range chickens from Uberlandia, Brazil

被引：11

作者：

Lopes, C. S. ^{[1
]}

Franco, P. S. ^{[2
]}

Silva, N. M. ^{[3
]}

Silva, D. A. O. ^{[1
]}

Ferro, E. A. V. ^{[2
]}

Pena, H. F. J. ^{[4
]}

Soares, R. M. ^{[4
]}

Gennari, S. M. ^{[4
]}

Mineo, J. R. ^{[1
]}

机构：

[1] Univ Fed Uberlandia, Dept Immunol Microbiol & Parasitol, Lab Immunoparasitol, Av Para 1720, BR-38400902 Uberlandia, MG, Brazil

[2] Univ Fed Uberlandia, Lab Immunophysiol Reprod, BR-38400902 Uberlandia, MG, Brazil

[3] Univ Fed Uberlandia, Dept Histol & Embryol, Immunopathol Lab, BR-38400902 Uberlandia, MG, Brazil

[4] Univ Sao Paulo, Sch Vet Med & Zootechny, Dept Vet Prevent Med & Anim Hlth, Parasit Dis Lab, BR-05508 Sao Paulo, Brazil

来源：

EPIDEMIOLOGY AND INFECTION | 2016年 / 144卷 / 09期

关键词：

Food protection; free-range chicken; phenotyping; tissue cyst; Toxoplasma gondii; UNITED-STATES; POPULATION-STRUCTURE; PARASITE; ANIMALS; HUMANS; FOOD; RECOMBINATION; ENCEPHALITIS; ANTIBODIES; PATHOGENS;

D O I：

10.1017/S0950268815003295

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The aim of this study was to determine the seroprevalence of Toxoplasma gondii infection in free-range chickens from Uberlandia, Minas Gerais state, Brazil, and characterize the genotypic and phenotypic features of two isolates of this parasite, considering the importance of these hosts in the epidemiology of toxoplasmosis. Serum samples from 108 free-range chickens were obtained from ten different districts, and submitted to the modified agglutination test (MAT) for the presence of anti-T. gondii antibodies, and brain and heart tissue samples from infected chickens were processed for mouse bioassay. An overall seroprevalence of 71.3% was found and antibody titres ranged from 16 to 4096. After confirmation of seropositivity by mouse bioassay, the determination of the T. gondii genotypes of two isolates was performed by PCR-RFLP, using primers for the following markers: SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, new SAG2, Apico and CS3. These T. gondii isolates, designated TgChBrUD1and TgChBrUD2, were obtained from heart samples of free-range chickens. The TgChBrUD1 isolate belonged to ToxoDB PCR-RFLP genotype 11 and the TgChBrUD2 isolate belonged to ToxoDB PCR-RFLP genotype 6. Both isolates demonstrated high virulence in a rodent model, with the TgChBrUD1 isolate able to induce brain cysts, in accord with its pattern of multiplication rates in human fibroblast culture. Taken together, these results reveal high prevalence of T. gondii infection in free-range chickens throughout Uberlandia, indicating an important degree of oocyst environmental contamination and the existence of considerable risk for T. gondii transmission to humans by consumption of free-range chicken as a food source.

引用

页码：1865 / 1875

页数：11

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