Overexpressed circPVT1 in oral squamous cell carcinoma promotes proliferation by serving as a miRNA sponge

被引:62
作者
He, Tianpeng [1 ]
Li, Xin [1 ]
Xie, Dongmei [1 ]
Tian, Lili [1 ]
机构
[1] China Japan Friendship Hosp, Dept Stomatol, 2 Yinghua East St, Beijing 100029, Peoples R China
基金
中国国家自然科学基金;
关键词
oral squamous cell carcinoma; circularPVT1; competing endogenous RNA; signal transducer and activator of transcription 3; cell proliferation; CIRCULAR RNA; NONCODING RNA; CANCER-PATIENTS; STAT3; HEAD; ASSOCIATION; EXPRESSION; SURVIVAL;
D O I
10.3892/mmr.2019.10615
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Circular RNAs (circRNAs) comprise a novel class of widespread non-coding RNAs that may regulate gene expression in eukaryotes. However, the characterization and function of circRNAs remain elusive in human cancer, including oral squamous cell carcinoma (OSCC). In this study, the expression level of circPVT1 in OSCC was detected and define its functional role in initiation and progression of OSCC. It was identified that circPVT1 was upregulated in OSCC cells and specimens. Knockdown of circPVT1 suppressed cell proliferation as evidenced by Cell Counting kit-8 assay and elevated Ki-67 expression. Mechanistically, it was demonstrated that circPVT1 possessed two targeting sites of microRNA (miRNA/miR)-125b and could effectively sponge miR-125b to release its downstream mRNA targets. Subsequently, the downstream target signal transducer and activator of transcription 3 (STAT3) was verified as a direct target of miR-125b and STAT3 expression was regulated by the circPVT1/miR-125b axis. CircPVT1 functioned as competing endogenous RNA (ceRNA) to increase the STAT3 level and cell proliferation through sponging miR-125b. In conclusion, circPVT1 regulates cell proliferation and may serve as a promising therapeutic target for OSCC patients. Therefore, silencing of circPVT1 could be a future direction to develop a novel treatment strategy.
引用
收藏
页码:3509 / 3518
页数:10
相关论文
共 38 条
  • [1] Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer
    Chen, Jie
    Li, Yan
    Zheng, Qiupeng
    Bao, Chunyang
    He, Jian
    Chen, Bin
    Lyu, Dongbin
    Zheng, Biqiang
    Xu, Yu
    Long, Ziwen
    Zhou, Ye
    Zhu, Huiyan
    Wang, Yanong
    He, Xianghuo
    Shi, Yingqiang
    Huang, Shenglin
    [J]. CANCER LETTERS, 2017, 388 : 208 - 219
  • [2] Regulation of circRNA biogenesis
    Chen, Ling-Ling
    Yang, Li
    [J]. RNA BIOLOGY, 2015, 12 (04) : 381 - 388
  • [3] Dai Chia-Yen, 2018, Oncotarget, V9, P11291, DOI 10.18632/oncotarget.24129
  • [4] Identifying and Characterizing circRNA-Protein Interaction
    Du, William W.
    Zhang, Chao
    Yang, Weining
    Yong, Tianqiao
    Awan, Faryal Mehwish
    Yang, Burton B.
    [J]. THERANOSTICS, 2017, 7 (17): : 4183 - 4191
  • [5] Screening circular RNA related to chemotherapeutic resistance in breast cancer
    Gao, Danfeng
    Zhang, Xiufen
    Liu, Beibei
    Meng, Dong
    Fang, Kai
    Guo, Zijian
    Li, Lihua
    [J]. EPIGENOMICS, 2017, 9 (09) : 1175 - 1188
  • [6] Applying the molecular biology and epigenetics of head and neck cancer in everyday clinical practice
    Glazer, Chad A.
    Chang, Steven S.
    Ha, Patrick K.
    Califano, Joseph A.
    [J]. ORAL ONCOLOGY, 2009, 45 (4-5) : 440 - 446
  • [7] An altered REDOX environment, assisted by over expression of fetal hemoglobins, protects from inflammatory colitis and reduces inflammatory cytokine expression (vol 50, pg 69, 2017)
    Gorczynski, R. M.
    Alexander, C.
    Brandenburg, K.
    Chen, Z.
    Heini, A.
    Neumann, D.
    Mach, J. P.
    Rietschel, E. T.
    Terskikh, A.
    Ulmer, A. J.
    Yu, Kai
    Zahringer, U.
    Khatri, I.
    [J]. INTERNATIONAL IMMUNOPHARMACOLOGY, 2018, 59 : 414 - 414
  • [8] Circular RNAs: Biogenesis, Function and Role in Human Diseases
    Greene, John
    Baird, Anne-Marie
    Brady, Lauren
    Lim, Marvin
    Gray, Steven G.
    McDermott, Raymond
    Finn, Stephen P.
    [J]. FRONTIERS IN MOLECULAR BIOSCIENCES, 2017, 4
  • [9] Potential of long non-coding RNAs in cancer patients: From biomarkers to therapeutic targets
    Gupta, Subash Chandra
    Tripathi, Yashoda Nandan
    [J]. INTERNATIONAL JOURNAL OF CANCER, 2017, 140 (09) : 1955 - 1967
  • [10] Circulating microRNAs in breast cancer: novel diagnostic and prognostic biomarkers
    Hamam, Rimi
    Hamam, Dana
    Alsaleh, Khalid A.
    Kassem, Moustapha
    Zaher, Waleed
    Alfayez, Musaad
    Aldahmash, Abdullah
    Alajez, Nehad M.
    [J]. CELL DEATH & DISEASE, 2017, 8 : e3045 - e3045