Electromagnetic needles with submicron pole tip radii for nanomanipulation of biomolecules and living cells

被引:56
作者
Matthews, BD [1 ]
LaVan, DA
Overby, DR
Karavitis, J
Ingber, DE
机构
[1] Harvard Univ, Childrens Hosp, Sch Med, Dept Pathol,Vasc Biol Program, Boston, MA 02115 USA
[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Pediat, Boston, MA 02115 USA
[3] Yale Univ, Dept Mech Engn, New Haven, CT 06511 USA
[4] Harvard Univ, Childrens Hosp, Sch Med, Dept Surg,Vasc Biol Program, Boston, MA 02115 USA
关键词
D O I
10.1063/1.1802383
中图分类号
O59 [应用物理学];
学科分类号
摘要
We describe the design and fabrication of a temperature-controlled electromagnetic microneedle (EMN) to generate custom magnetic field gradients for biomedical and biophysical applications. An electropolishing technique was developed to sharpen the EMN pole tip to any desired radius between 100 nm and 20 mum. The EMN can be used to apply strong static or dynamic forces (>50 nN) to micrometer- or nanometer-sized magnetic beads without producing significant heating or needle movement. Large tip radii (20 mum) allow magnetic force application to multiple magnetic beads over a large area, while small radii (0.1-6 mum) can be used to selectively pull or capture single magnetic beads from within a large population of similar particles. The customizable EMN is thus well suited for micro- and nanomanipulation of magnetic particles linked to biomolecules or living cells. (C) 2004 American Institute of Physics.
引用
收藏
页码:2968 / 2970
页数:3
相关论文
共 13 条
[1]   Analysis of cell mechanics in single vinculin-deficient cells using a magnetic tweezer [J].
Alenghat, FJ ;
Fabry, B ;
Tsai, KY ;
Goldmann, WH ;
Ingber, DE .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 277 (01) :93-99
[2]   Scanning probe electromagnetic tweezers [J].
Barbic, M ;
Mock, JJ ;
Gray, AP ;
Schultz, S .
APPLIED PHYSICS LETTERS, 2001, 79 (12) :1897-1899
[3]   Local measurements of viscoelastic parameters of adherent cell surfaces by magnetic bead microrheometry [J].
Bausch, AR ;
Ziemann, F ;
Boulbitch, AA ;
Jacobson, K ;
Sackmann, E .
BIOPHYSICAL JOURNAL, 1998, 75 (04) :2038-2049
[4]   Twisting integrin receptors increases endothelin-1 gene expression in endothelial cells [J].
Chen, JX ;
Fabry, B ;
Schiffrin, EL ;
Wang, N .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2001, 280 (06) :C1475-C1484
[5]   Integrin binding and mechanical tension induce movement of mRNA and ribosomes to focal adhesions [J].
Chicurel, ME ;
Singer, RH ;
Meyer, CJ ;
Ingber, DE .
NATURE, 1998, 392 (6677) :730-733
[6]   Scaling the microrheology of living cells [J].
Fabry, B ;
Maksym, GN ;
Butler, JP ;
Glogauer, M ;
Navajas, D ;
Fredberg, JJ .
PHYSICAL REVIEW LETTERS, 2001, 87 (14) :148102/1-148102/4
[7]   Using biofunctional magnetic nanoparticles to capture vancomycin-resistant enterococci and other gram-positive bacteria at ultralow concentration [J].
Gu, HW ;
Ho, PL ;
Tsang, KWT ;
Wang, L ;
Xu, B .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (51) :15702-15703
[8]   A NOVEL REMOTE-SENSING ISOMETRIC FORCE TRANSDUCER FOR MICROMECHANICS STUDIES [J].
GUILFORD, WH ;
GORE, RW .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (03) :C700-C707
[9]   Mechanical properties of individual focal adhesions probed with a magnetic microneedle [J].
Matthews, BD ;
Overby, DR ;
Alenghat, FJ ;
Karavitis, J ;
Numaguchi, Y ;
Allen, PG ;
Ingber, DE .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 313 (03) :758-764
[10]   Mechanical control of cyclic AMP signalling and gene transcription through integrins [J].
Meyer, CJ ;
Alenghat, FJ ;
Rim, P ;
Fong, JHJ ;
Fabry, B ;
Ingber, DE .
NATURE CELL BIOLOGY, 2000, 2 (09) :666-668