Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements - in vitro

被引：4

作者：

Galic, Elizabeta ^{[1
]}

Tadin, Antonija ^{[2
]}

Galic, Nada ^{[3
]}

Kasuba, Vilena ^{[4
]}

Mladinic, Marin ^{[4
]}

Rozgaj, Ruzica ^{[4
]}

Biocina-Lukenda, Dolores ^{[2
]}

Galic, Ivan ^{[2
]}

Zeljezic, Davor ^{[4
]}

机构：

[1] Univ Split, Sch Med, Hlth Ctr Split Dalmatia Cty, Split 21000, Croatia

[2] Univ Split, Sch Med, Study Dent Med, Split 21000, Croatia

[3] Univ Zagreb, Sch Dent Med, Dept Endodont & Restorat Dent Med, Zagreb 41000, Croatia

[4] Inst Med Res & Occupat Hlth, Mutagenesis Unit, Zagreb, Croatia

来源：

ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY | 2014年 / 65卷 / 02期

关键词：

biocompatibility; cytotoxicity; DNA damage; genotoxicity; lymphocytes; HUMAN-LYMPHOCYTES; DNA-DAMAGE; ZINC-OXIDE; CYTOTOXICITY; BIOCOMPATIBILITY; GENOTOXICITY; CELLS; VIVO;

D O I：

10.2478/10004-1254-65-2014-2392

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The purpose of this study was to evaluate the genotoxic potential of components leached from two conventional self-curing glass-ionomer cements (Fuji IX and Ketac Molar), and light-curing, resin modified glass-ionomer cements (Vitrebond, Fuji II LC). Evaluation was performed on human lymphocytes using alkaline and hOGG1 modified comet, and micronucleus assays. Each material, polymerised and unpolymerised, was eluted in extracellular saline (1 cm(2) mL(-1)) for 1 h, 1 day, and 5 days. Cultures were treated with eluates using final dilutions of 10(-2), 10(-3), and 10(-4). Alkaline comet assay did not detect changes in DNA migration of treated cells regardless of the ionomer tested, polymerisation state, and elution duration. Glass ionomers failed to significantly influence micronucleus frequency. No oxidative DNA damage in treated lymphocytes was observed using hOGG1 modified comet assay. Obtained results indicate high biocompatibility of all tested materials used in the study under experimental conditions.

引用

页码：179 / 188

页数：10

共 31 条

[1]

[Anonymous], 2009, BASIC ASPECT BIOCOMP

[2]

[Anonymous], 1996, 1099312 ISO 12

[3] Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line [J].

Aranha, Andreza M. F. ;

Giro, Elisa M. A. ;

Souza, Pedro P. C. ;

Hebling, Josimeri ;

Costa, Carlos A. de Souza .

DENTAL MATERIALS, 2006, 22 (09) :864-869

[4] Genotoxic and cytotoxic effects of different types of dental cement on normal cultured human lymphocytes [J].

Bakopoulou, A. ;

Mourelatos, D. ;

Tsiftsoglou, A. S. ;

Giassin, N. P. ;

Mioglou, E. ;

Garefis, P. .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2009, 672 (02) :103-112

[5] Resin-modified Glass-ionomer Setting Reaction Competition [J].

Berzins, D. W. ;

Abey, S. ;

Costache, M. C. ;

Wilkie, C. A. ;

Roberts, H. W. .

JOURNAL OF DENTAL RESEARCH, 2010, 89 (01) :82-86

[6] THE EFFECTS OF HYALURONIC ACID, CALCIUM HYDROXIDE, AND DENTIN ADHESIVE ON RAT ODONTOBLASTS AND FIBROBLASTS [J].

Bogovic, Ana ;

Nizetic, Jana ;

Galic, Nada ;

Zeljezic, Davor ;

Micek, Vedran ;

Mladinic, Mann .

ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY, 2011, 62 (02) :155-161

[7] The comet assay for DNA damage and repair - Principles, applications, and limitations [J].

Collins, AR .

MOLECULAR BIOTECHNOLOGY, 2004, 26 (03) :249-261

[8] In vitro cytotoxicity of five glass-ionomer cements [J].

Costa, CAD ;

Hebling, J ;

Garcia-Godoy, F ;

Hanks, CT .

BIOMATERIALS, 2003, 24 (21) :3853-3858

[9] Gel phase formation at resin-modified glass-ionomer/tooth interfaces [J].

Coutinho, E. ;

Yoshida, Y. ;

Inoue, S. ;

Fukuda, R. ;

Snauwaert, J. ;

Nakayama, Y. ;

De Munck, J. ;

Lambrechts, P. ;

Suzuki, K. ;

Van Meerbeek, B. .

JOURNAL OF DENTAL RESEARCH, 2007, 86 (07) :656-661

[10]

Do Nascimento ABL, 2000, AM J DENT, V13, P28

← 1 2 3 4 →