Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis

被引：103

作者：

Domenis, Rossana ^{[1
]}

Zanutel, Rossella ^{[1
]}

Caponnetto, Federica ^{[1
]}

Toffoletto, Barbara ^{[1
]}

Cifu, Adriana ^{[1
]}

Pistis, Cinzia ^{[1
]}

Di Benedetto, Paolo ^{[2
]}

Causero, Araldo ^{[2
]}

Pozzi, Massimo ^{[3
]}

Bassini, Fabrizio ^{[3
]}

Fabris, Martina ^{[1
,4
]}

Niazi, Kayvan R. ^{[5
]}

Soon-Shiong, Patrick ^{[5
]}

Curcio, Francesco ^{[1
,4
]}

机构：

[1] Univ Udine, Dipartimento Area Med DAME, Udine, Italy

[2] Azienda Sanit Univ Integrata Udine, Clin Ortoped, Udine, Italy

[3] Azienda Assistenza Sanit, Dipartimento Ortopedia & Traumatol, 3 Alto Friuli, Tolmezzo, Italy

[4] Azienda Sanita Univ Integrata Udine, Ist Patol Clin, Udine, Italy

[5] NantBioSci Inc, Culver City, CA 90232 USA

来源：

MEDIATORS OF INFLAMMATION | 2017年 / 2017卷

关键词：

EXTRACELLULAR VESICLES; MATRIX-METALLOPROTEINASE; GRO-ALPHA; TNF-ALPHA; MICROPARTICLES; ANGIOGENESIS; IL-1-BETA; CYTOKINES; PATHOGENESIS; INFLAMMATION;

D O I：

10.1155/2017/4814987

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The purpose of this study is to characterize synovial fluid- (SF-) derived exosomes of patients with gonarthrosis comparing two methods of isolation and to investigate their immune regulatory properties. Extracellular vesicles (EVs) have been isolated from inflamed SF by polymer precipitation method and quantified by Exocet kit and by nanoparticle tracking analysis. Vesicles expressed all the specific exosomal markers by immunoblot and FACS. After isolation with Exoquick, a relevant contamination by immune complexes was detected, which required further magnetic bead-based purification to remove. SF- derived exosomes significantly stimulated the release of several inflammatory cytokines and chemokines and metalloproteinases by M1 macrophages but did not influence the expression of CD80 and CD86 costimulatory molecules. In conclusion, we characterized purified exosomes isolated from inflamed SF and demonstrate that purified exosomes are functionally active in their ability to stimulate the release of proinflammatory factors from M1 macrophages. Our data indicate that SF-derived exosomes from gonarthrosis patients play a role in disease progression.

引用

页数：11

共 38 条

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