An SNP-Based Genetic Map and QTL Mapping for Growth Traits in the Red-Spotted Grouper (Epinephelus akaara)

被引：12

作者：

Wang, Xiang ^{[1
,2
]}

Jiang, Shoujia ^{[3
]}

Zheng, Leyun ^{[4
]}

Xiao, Ling ^{[5
,6
]}

Zhang, Xinhui ^{[3
]}

Wang, Dengdong ^{[5
,6
]}

Li, Shuisheng ^{[5
,6
,7
]}

Shi, Qiong ^{[3
]}

Wu, Shuiqing ^{[4
]}

Lin, Haoran ^{[2
,5
,6
,7
]}

You, Xinxin ^{[3
]}

Zhang, Yong ^{[2
,5
,6
,7
]}

机构：

[1] SOA, South China Sea Marine Survey & Technol Ctr, Guangzhou 510275, Guangdong, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266373, Shandong, Peoples R China

[3] BGI Acad Marine Sci, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ A, BGI Marine,BGI, Shenzhen 518083, Guangdong, Peoples R China

[4] Fisheries Res Inst Fujian, Xiamen 361012, Fujian, Peoples R China

[5] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

[6] Sun Yat Sen Univ, Guangdong Prov Engn Technol Res Ctr Hlth Breeding, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

[7] Guangdong Ocean Univ, Fisheries Coll, Southern Marine Sci & Engn Guangdong Lab ZhanJian, Zhanjiang 524088, Peoples R China

来源：

GENES | 2019年 / 10卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Epinephelus akaara; genetic map; RAD; growth-related traits; QTL; network analysis; CYTOCHROME-OXIDASE ACTIVITY; LINKAGE MAP; DE-NOVO; CONSTRUCTION; HORMONE; LOCI; SOMATOSTATIN; YELLOW;

D O I：

10.3390/genes10100793

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The red-spotted grouper (Epinephelus akaara) is one of the most commercially important aquatic species in China. However, its seedstock has low larval survival rates, and its stability is confronted with the danger of overexploitation. In this study, a high-density genetic map was constructed using 3435 single nucleotide polymorphisms (SNPs) from 142 first generation (F-1) full-sib offspring and two parents of a red-spotted grouper population. The total genetic length of the map was 2300.12 cM with an average intermarker distance of 0.67 cM. Seventeen genome-wide significant quantitative trait loci (QTLs) for growth-related traits were detected on 24 linkage groups, including 5 QTLs for full length, 7 QTLs for body length, and 5 QTLs for body weight. The contribution values of explained phenotypic variance ranged from 10.7% to 12.9%. Moreover, 13 potential candidate genes for growth-related traits were identified. Collectively, these findings will be useful for conducting marker-assisted selection of the red-spotted grouper in future studies.

引用

页数：16

共 78 条

[51]

Silva Luciano Da Costa E, 2012, Methods Mol Biol, V871, P75, DOI 10.1007/978-1-61779-785-9_6

[52] RELATIONSHIP OF CYTOCHROME-OXIDASE ACTIVITY TO VERTEBRATE TOTAL AND ORGAN OXYGEN-CONSUMPTION [J].

SIMON, LM ;

ROBIN, ED .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1971, 2 (11) :569-&

[53] Mapping Genetic Loci for Quantitative Traits of Golden Shell Color, Mineral Element Contents, and Growth-Related Traits in Pacific Oyster (Crassostrea gigas) [J].

Song, Junlin ;

Li, Qi ;

Yu, Yong ;

Wan, Sai ;

Han, Lichen ;

Du, Shaojun .

MARINE BIOTECHNOLOGY, 2018, 20 (05) :666-675

[54] Construction of High-Density Genetic Linkage Maps and Mapping of Growth-Related Quantitative Trail Loci in the Japanese Flounder (Paralichthys olivaceus) [J].

Song, Wentao ;

Pang, Renyi ;

Niu, Yuze ;

Gao, Fengtao ;

Zhao, Yongwei ;

Zhang, Jing ;

Sun, Jian ;

Shao, Changwei ;

Liao, Xiaolin ;

Wang, Lei ;

Tian, Yongsheng ;

Chen, Songlin .

PLOS ONE, 2012, 7 (11)

[55] Construction of a high-density linkage map and mapping of sex determination and growth-related loci in the mandarin fish (Siniperca chuatsi) [J].

Sun, Chengfei ;

Niu, Yongchaox ;

Ye, Xing ;

Dong, Junjian ;

Hu, Wushu ;

Zeng, Qingkai ;

Chen, Zhihang ;

Tian, Yuanyuan ;

Zhang, Jin ;

Lu, Maixin .

BMC GENOMICS, 2017, 18

[56] The STRING database in 2017: quality-controlled protein-protein association networks, made broadly accessible [J].

Szklarczyk, Damian ;

Morris, John H. ;

Cook, Helen ;

Kuhn, Michael ;

Wyder, Stefan ;

Simonovic, Milan ;

Santos, Alberto ;

Doncheva, Nadezhda T. ;

Roth, Alexander ;

Bork, Peer ;

Jensen, Lars J. ;

von Mering, Christian .

NUCLEIC ACIDS RESEARCH, 2017, 45 (D1) :D362-D368

[57] Sialidase NEU4 Hydrolyzes Polysialic Acids of Neural Cell Adhesion Molecules and Negatively Regulates Neurite Formation by Hippocampal Neurons [J].

Takahashi, Kohta ;

Mitoma, Junya ;

Hosono, Masahiro ;

Shiozaki, Kazuhiro ;

Sato, Chihiro ;

Yamaguchi, Kazunori ;

Kitajima, Ken ;

Higashi, Hideyoshi ;

Nitta, Kazuo ;

Shima, Hiroshi ;

Miyagi, Taeko .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (18) :14816-14826

[58] Construction and Annotation of a High Density SNP Linkage Map of the Atlantic Salmon (Salmo salar) Genome [J].

Tsai, Hsin Y. ;

Robledo, Diego ;

Lowe, Natalie R. ;

Bekaert, Michael ;

Taggart, John B. ;

Bron, James E. ;

Houston, Ross D. .

G3-GENES GENOMES GENETICS, 2016, 6 (07) :2173-2179

[59] Genotyping-by-sequencing for construction of a new genetic linkage map and QTL analysis of growth-related traits in Pacific bluefin tuna [J].

Uchino, Tsubasa ;

Hosoda, Erina ;

Nakamura, Yoji ;

Yasuike, Motoshige ;

Mekuchi, Miyuki ;

Sekino, Masashi ;

Fujiwara, Atushi ;

Sugaya, Takuma ;

Tanaka, Yosuke ;

Kumon, Kazunori ;

Agawa, Yasuo ;

Sawada, Yoshifumi ;

Sano, Motohiko ;

Sakamoto, Takashi .

AQUACULTURE RESEARCH, 2018, 49 (03) :1293-1301

[60] ACCURACY OF MAPPING QUANTITATIVE TRAIT LOCI IN AUTOGAMOUS SPECIES [J].

VANOOIJEN, JW .

THEORETICAL AND APPLIED GENETICS, 1992, 84 (7-8) :803-811

← 1 2 3 4 5 6 7 8 →