An SNP-Based Genetic Map and QTL Mapping for Growth Traits in the Red-Spotted Grouper (Epinephelus akaara)

被引：12

作者：

Wang, Xiang ^{[1
,2
]}

Jiang, Shoujia ^{[3
]}

Zheng, Leyun ^{[4
]}

Xiao, Ling ^{[5
,6
]}

Zhang, Xinhui ^{[3
]}

Wang, Dengdong ^{[5
,6
]}

Li, Shuisheng ^{[5
,6
,7
]}

Shi, Qiong ^{[3
]}

Wu, Shuiqing ^{[4
]}

Lin, Haoran ^{[2
,5
,6
,7
]}

You, Xinxin ^{[3
]}

Zhang, Yong ^{[2
,5
,6
,7
]}

机构：

[1] SOA, South China Sea Marine Survey & Technol Ctr, Guangzhou 510275, Guangdong, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266373, Shandong, Peoples R China

[3] BGI Acad Marine Sci, Shenzhen Key Lab Marine Genom, Guangdong Prov Key Lab Mol Breeding Marine Econ A, BGI Marine,BGI, Shenzhen 518083, Guangdong, Peoples R China

[4] Fisheries Res Inst Fujian, Xiamen 361012, Fujian, Peoples R China

[5] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

[6] Sun Yat Sen Univ, Guangdong Prov Engn Technol Res Ctr Hlth Breeding, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

[7] Guangdong Ocean Univ, Fisheries Coll, Southern Marine Sci & Engn Guangdong Lab ZhanJian, Zhanjiang 524088, Peoples R China

来源：

GENES | 2019年 / 10卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Epinephelus akaara; genetic map; RAD; growth-related traits; QTL; network analysis; CYTOCHROME-OXIDASE ACTIVITY; LINKAGE MAP; DE-NOVO; CONSTRUCTION; HORMONE; LOCI; SOMATOSTATIN; YELLOW;

D O I：

10.3390/genes10100793

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The red-spotted grouper (Epinephelus akaara) is one of the most commercially important aquatic species in China. However, its seedstock has low larval survival rates, and its stability is confronted with the danger of overexploitation. In this study, a high-density genetic map was constructed using 3435 single nucleotide polymorphisms (SNPs) from 142 first generation (F-1) full-sib offspring and two parents of a red-spotted grouper population. The total genetic length of the map was 2300.12 cM with an average intermarker distance of 0.67 cM. Seventeen genome-wide significant quantitative trait loci (QTLs) for growth-related traits were detected on 24 linkage groups, including 5 QTLs for full length, 7 QTLs for body length, and 5 QTLs for body weight. The contribution values of explained phenotypic variance ranged from 10.7% to 12.9%. Moreover, 13 potential candidate genes for growth-related traits were identified. Collectively, these findings will be useful for conducting marker-assisted selection of the red-spotted grouper in future studies.

引用

页数：16

共 78 条

[1]

Andersen Synne M, 2016, Front Biosci (Elite Ed), V8, P143

[2] Harnessing the power of RADseq for ecological and evolutionary genomics [J].

Andrews, Kimberly R. ;

Good, Jeffrey M. ;

Miller, Michael R. ;

Luikart, Gordon ;

Hohenlohe, Paul A. .

NATURE REVIEWS GENETICS, 2016, 17 (02) :81-92

[3] Genetic Map Construction and Quantitative Trait Locus (QTL) Detection of Growth-Related Traits in Litopenaeus vannamei for Selective Breeding Applications [J].

Andriantahina, Farafidy ;

Liu, Xiaolin ;

Huang, Hao .

PLOS ONE, 2013, 8 (09)

[4] Construction of the High-Density Genetic Linkage Map and Chromosome Map of Large Yellow Croaker (Larimichthys crocea) [J].

Ao, Jingqun ;

Li, Jia ;

You, Xinxin ;

Mu, Yinnan ;

Ding, Yang ;

Mao, Kaiqiong ;

Bian, Chao ;

Mu, Pengfei ;

Shi, Qiong ;

Chen, Xinhua .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (11) :26237-26248

[5] High-Density Linkage Map and QTLs for Growth in Snapper (Chrysophrys auratus) [J].

Ashton, David T. ;

Ritchie, Peter A. ;

Wellenreuther, Maren .

G3-GENES GENOMES GENETICS, 2019, 9 (04) :1027-1035

[6] Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers [J].

Baird, Nathan A. ;

Etter, Paul D. ;

Atwood, Tressa S. ;

Currey, Mark C. ;

Shiver, Anthony L. ;

Lewis, Zachary A. ;

Selker, Eric U. ;

Cresko, William A. ;

Johnson, Eric A. .

PLOS ONE, 2008, 3 (10)

[7] Stacks: Building and Genotyping Loci De Novo From Short-Read Sequences [J].

Catchen, Julian M. ;

Amores, Angel ;

Hohenlohe, Paul ;

Cresko, William ;

Postlethwait, John H. .

G3-GENES GENOMES GENETICS, 2011, 1 (03) :171-182

[8] De Novo Transcriptome Characterization and Growth-Related Gene Expression Profiling of Diploid and Triploid Bighead Catfish (Clarias macrocephalus Gunther, 1864) [J].

Chatchaiphan, Satid ;

Srisapoome, Prapansak ;

Kim, Jin-Hyoung ;

Devlin, Robert H. ;

Na-Nakorn, Uthairat .

MARINE BIOTECHNOLOGY, 2017, 19 (01) :36-48

[9] Construction of a Microsatellites-Based Linkage Map for the White Grouper (Epinephelus aeneus) [J].

Dor, Lior ;

Shirak, Andrey ;

Gorshkov, Sergei ;

Band, Mark R. ;

Korol, Abraham ;

Ronin, Yefim ;

Curzon, Arie ;

Hulata, Gideon ;

Seroussi, Eyal ;

Ron, Micha .

G3-GENES GENOMES GENETICS, 2014, 4 (08) :1455-1464

[10] Evaluation of the 2b-RAD method for genomic selection in scallop breeding [J].

Dou, Jinzhuang ;

Li, Xue ;

Fu, Qiang ;

Jiao, Wenqian ;

Li, Yangping ;

Li, Tianqi ;

Wang, Yangfan ;

Hu, Xiaoli ;

Wang, Shi ;

Bao, Zhenmin .

SCIENTIFIC REPORTS, 2016, 6

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