Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida)

被引：24

作者：

Cermakova, Petra

Verner, Zdenek

Man, Petr

Lukes, Julius

Horvath, Anton

机构：

[1] Comenius Univ, Fac Nat Sci, Dept Biochem, Bratislava 84215, Slovakia

[2] Univ S Bohemia, Czech Acad Sci, Inst Parasitol, Biol Ctr, Ceske Budejovice, Czech Republic

[3] Univ S Bohemia, Fac Biol, Ceske Budejovice, Czech Republic

[4] Acad Sci Czech Republ, Inst Microbiol, Prague, Czech Republic

来源：

FEBS JOURNAL | 2007年 / 274卷 / 12期

关键词：

complex I; NADH dehydrogenase; Phytomonas; respiratory chain; trypanosomatid;

D O I：

10.1111/j.1742-4658.2007.05847.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NADH dehydrogenase activity was characterized in the mitochondrial lysates of Phytomonas serpens, a trypanosomatid flagellate parasitizing plants. Two different high molecular weight NADH dehydrogenases were characterized by native PAGE and detected by direct in-gel activity staining. The association of NADH dehydrogenase activities with two distinct multisubunit complexes was revealed in the second dimension performed under denaturing conditions. One subunit present in both complexes cross-reacted with the antibody against the 39 kDa subunit of bovine complex I. Out of several subunits analyzed by MS, one contained a domain characteristic for the LYR family subunit of the NADH:ubiquinone oxidoreductases. Spectrophotometric measurement of the NADH:ubiquinone 10 and NADH:ferricyanide dehydrogenase activities revealed their different sensitivities to rotenone, piericidin, and diphenyl iodonium.

引用

页码：3150 / 3158

页数：9

共 38 条

[1] The presence of rotenone-sensitive NADH dehydrogenase in the long slender bloodstream and the procyclic forms of Trypanosoma brucei brucei
Beattie, DS
Howton, MM
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 241 (03): : 888 - 894
[2] Import of a constitutively expressed protein into mitochondria from procyclic and bloodstream forms of Trypanosoma brucei
Bertrand, KI
Hajduk, SL
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2000, 106 (02) : 249 - 260
[3] Functional characterization and target validation of alternative complex I of Plasmodium falciparum mitochondria
Biagini, GA
Viriyavejakul, P
O'Neill, PM
Bray, PG
Ward, SA
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (05) : 1841 - 1851
[4] Energy converting NADH:Quinone oxidoreductase (Complex I)
Brandt, Ulrich
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 2006, 75 : 69 - 92
[5] Definition of the nuclear encoded protein composition of bovine heart mitochondrial complex I - Identification of two new subunits
Carroll, J
Shannon, RJ
Fearnley, IM
Walker, JE
Hirst, J
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (52) : 50311 - 50317
[6] Christmas PB, 2000, FEMS MICROBIOL LETT, V183, P225, DOI 10.1111/j.1574-6968.2000.tb08962.x
[7] SUCCINATE-DEPENDENT METABOLISM IN TRYPANOSOMA-CRUZI EPIMASTIGOTES
DENICOLASEOANE, A
RUBBO, H
PRODANOV, E
TURRENS, JF
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 54 (01) : 43 - 50
[8] Inhibitors of NADH-ubiquinone reductase: an overview
Esposti, MD
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1364 (02): : 222 - 235
[9] Identification of a gene encoding a 54 kDa alternative NADH dehydrogenase in Trypanosoma brucei
Fang, J
Beattie, DS
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2003, 127 (01) : 73 - 77
[10] Rotenone-insensitive NADH dehydrogenase is a potential source of superoxide in procyclic Trypanosoma brucei mitochondria
Fang, J
Beattie, DS
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2002, 123 (02) : 135 - 142

← 1 2 3 4 →