Lysophosphatidic acid induces STAT3 phosphorylation and ovarian cancer cell motility: Their inhibition by curcumin

被引：78

作者：

Seo, Ji Hye ^{[1
]}

Jeong, Kang Jin ^{[1
]}

Oh, Woo Jin ^{[1
]}

Sul, Hae Jeong ^{[1
]}

Sohn, Jang Sihn ^{[1
]}

Kim, Yong Kee ^{[2
]}

Cho, Do Yeun ^{[1
]}

Kang, Jae Ku ^{[1
]}

Park, Chang Gyo ^{[1
]}

Lee, Hoi Young ^{[1
]}

机构：

[1] Konyang Univ, Coll Med, Myunggok Med Res Inst, Taejon 302718, South Korea

[2] Kwandong Univ, Coll Med, Dept Pharmacol, Kangnung 210701, South Korea

来源：

CANCER LETTERS | 2010年 / 288卷 / 01期

关键词：

Curcumin; Lysophosphatidic acid; Ovarian cancer; Motility; Signal transducers; Activators of transcription 3; KAPPA-B; CARCINOMA-CELLS; ACTIVATION; EXPRESSION; PROLIFERATION; MIGRATION; INVASION; GROWTH;

D O I：

10.1016/j.canlet.2009.06.023

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Lysophosphatidic acid (LPA) is a biolipid that stimulates tumor cell invasion and metastasis. In this report, we determined the role of signal transducers and activators of transcription 3 (STAT3) and the effect of a chemopreventive agent, curcumin, on LPA-induced ovarian cancer cell motility. LPA phosphorylated STAT3 in a dose-dependent manner. Treatment of cells with a JAK/STAT inhibitor, AG490. inhibited LPA-induced cell motility. In contrast, transfection of a constitutively active form of STAT3 induced ovarian cancer cell motility. LPA also stimulated interleukin (IL)-6 and IL-8 secretion, which results in STAT3 phosphorylation. Treatment of the cells with curcumin inhibited LPA-induced IL-6 and IL-8 secretion and STAT3 phosphorylation, leading to blocked ovarian cancer cell motility. Collectively, the present study shows the critical role of STAT3 in ovarian cancer cell motility and that this process can be prevented by curcumin. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：50 / 56

页数：7

共 29 条

[21] Curcumin: The story so far [J].

Sharma, RA ;

Gescher, AJ ;

Steward, WP .

EUROPEAN JOURNAL OF CANCER, 2005, 41 (13) :1955-1968

[22] Activated signal transducer and activator of transcription (STAT) 3: Localization in focal adhesions and function in ovarian cancer cell motility [J].

Silver, DL ;

Naora, H ;

Liu, JS ;

Cheng, WJ ;

Montell, DJ .

CANCER RESEARCH, 2004, 64 (10) :3550-3558

[23] ACTIVATION OF TRANSCRIPTION FACTOR NF-KAPPA-B IS SUPPRESSED BY CURCUMIN (DIFERULOLYLMETHANE) [J].

SINGH, S ;

AGGARWAL, BB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (42) :24995-25000

[24] Lysophosphatidic acid enhances epithelial ovarian carcinoma invasion through the increased expression of interleukin-8 [J].

So, J ;

Navari, J ;

Wang, FQ ;

Fishman, DA .

GYNECOLOGIC ONCOLOGY, 2004, 95 (02) :314-322

[25]

STRACKE ML, 1992, J BIOL CHEM, V267, P2524

[26]

van Leeuwen FN, 2003, BIOCHEM SOC T, V31, P1209

[27] Curcumin inhibits neurotensin-mediated interleukin-8 production and migration of HCT116 human colon cancer cells [J].

Wang, Xiaofu ;

Wang, Qingding ;

Ives, Kirk L. ;

Evers, B. Mark .

CLINICAL CANCER RESEARCH, 2006, 12 (18) :5346-5355

[28] The Interleukin-8 Pathway in Cancer [J].

Waugh, David J. J. ;

Wilson, Catherine .

CLINICAL CANCER RESEARCH, 2008, 14 (21) :6735-6741

[29] Malignant effusions contain lysophosphatidic acid (LPA) like activity [J].

Westermann, AM ;

Havik, E ;

Postma, FR ;

Beijnen, JH ;

Dalesio, O ;

Moolenaar, WH ;

Rodenhuis, S .

ANNALS OF ONCOLOGY, 1998, 9 (04) :437-442

← 1 2 3 →