EFFECTS OF NITROGEN STARVATION ON EFFECTOR REPERTOIRE OF PROTEINS SECRETED FROM PHYTOPHTHORA INFESTANS (MONT.) DE BARY

Phytophthora infestans strain NOD-1 was used as experimental material to extract its secreted proteins after nitrogen starvation treatment, and label-free proteomics technology was used to investigate the expression difference of the secreted proteins after various lengths of treatment. By using bioinformatic tools, functional annotation and metabolic pathway analyses were performed for the differentially expressed secreted proteins. According to present results, the expression level of cytoplasmic and extracellular effector proteins increased with the prolongation of treatment time, and also more effector proteins were induced. The expression level of RXLR-like effectors gradually increased under complete culture conditions, and CRN-like effector proteins began to express slightly after 24 days. By contrast, the expression level of CRN-like effector protein increased gradually under nitrogen starvation conditions, and RXLR-like effector protein began to express slightly at 24 days. In addition, except for one small cysteine-rich protein SCR91, all other extracellular effector proteins were highly expressed under complete culture conditions. This study provides a theoretical basis for a more comprehensive understanding of the pathogenesis of P. infestans through functional studies of effector proteins.