Retrocyclin 2: a new therapy against avian influenza H5N1 virus in vivo and vitro

被引：16

作者：

Liang, Qing-long ^{[1
,2
]}

Zhou, Kai ^{[1
,2
]}

He, Hong-xuan ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Zool, Natl Res Ctr Wildlife Born Dis, Key Lab Anim Ecol & Conservat Biol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 03期

关键词：

Avian influenza virus; Chicken embryos; H5N1; virus; Influenza virus; MDCK cells; Retrocyclin; 2; THETA-DEFENSINS; ALPHA-DEFENSINS; LEUKOCYTES; INFECTION; PROTEINS; PEPTIDE; ENTRY; HIV-1; CELLS;

D O I：

10.1007/s10529-009-0167-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We tested the ability of retrocyclin 2, a type of theta defensin, to protect cells and chicken embryos from infection by H5N1 highly pathogenic avian influenza virus. A gene fragment of retrocyclin 2 was designed based on the protein sequence of retrocyclin 2 and cloned into the eukaryotic expression vector pcDNA4.01 (HismaxA), named pcDNA4-RC2. The expression vector pcDNA4-RC2 protected MDCK cells and chicken embryos from infection by the H5N1 virus through inhibition of virus replication and viral mRNA transcription. Retrocyclin 2 is therefore effective in preventing H5N1 virus infection in vivo and in vitro and could be considered as a new therapy for H5N1 influenza and other diseases.

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页码：387 / 392

页数：6

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