Meiotic spindle size is a strong indicator of human oocyte quality

被引：29

作者：

Tomari, Hiroyuki ^{[1
]}

Honjo, Ko ^{[1
]}

Kunitake, Katsuko ^{[1
]}

Aramaki, Natsumi ^{[1
]}

Kuhara, Saori ^{[1
]}

Hidaka, Naomi ^{[1
]}

Nishimura, Kayoko ^{[1
]}

Nagata, Yumi ^{[1
]}

Horiuchi, Toshitaka ^{[2
]}

机构：

[1] IVF Nagata Clin, Fukuoka, Fukuoka, Japan

[2] Prefectural Univ Hiroshima, Grad Sch Comprehens Sci Res, Hiroshima, Japan

来源：

REPRODUCTIVE MEDICINE AND BIOLOGY | 2018年 / 17卷 / 03期

关键词：

assisted reproductive technology; blastocyst; fertilization; intracytoplasmic sperm injection; meiotic spindle;

D O I：

10.1002/rmb2.12100

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

PurposeTo investigate the relationship between the meiotic spindle size in human metaphase II oocytes and embryo developmental potential after intracytoplasmic sperm injection (ICSI). MethodsAnalyzed were 1302 oocytes with a visible meiotic spindle from 281 patients aged under 40years undergoing ICSI cycles. The meiotic spindle was imaged by using PolScope before ICSI. The oocytes were classified into three groups, according to spindle size: group A (<90m(2)), group B (90-120m(2)), and group C (>120m(2)). ResultsOverall, 389 (29.9%) oocytes were classified into group A, 662 (50.8%) into group B, and 251 (19.3%) into group C. The fertilization rate of the group B oocytes was significantly higher than for the A and C oocytes. The blastocyst formation rate in group B was significantly higher than in group A. In addition, the pregnancy rate in group B was significantly higher than in the other two groups. ConclusionThe oocytes with a spindle size of 90-120m(2) showed higher fertilization, blastocyst formation, and clinical pregnancy rates than those with larger or smaller spindles. The measurement of the meiotic spindle size thus has a positive predictive value for identifying human embryo developmental potential clinically.

引用

页码：268 / 274

页数：7

共 28 条

[1] Spindle imaging: a new marker for optimal timing of ICSI? [J].