Characterisation of recombinant isoforms of birch pollen allergen Bet v 1

被引：0
|
作者：
Spangfort, MD
Ipsen, H
Sparholt, SH
AasmulOlsen, S
Osmark, P
Poulsen, FM
Larsen, M
Mortz, E
Roepstorff, P
Larsen, JN
机构：
来源：
NEW HORIZONS IN ALLERGY IMMUNOTHERAPY | 1996年 / 409卷
关键词：
D O I：
暂无
中图分类号：
R392 [医学免疫学];
学科分类号：
100102 ;
摘要：
Three isoforms of the major birch pollen allergen, Bet v, 1 from Betula verrucosa have been expressed as recombinant proteins in E. coli and purified. The immunochemical properties of recombinant isoforms (rBet v 1) differed on immunoblots when compared using Mabs and birch pollen allergic patients serum IgE. 2-D gel analysis showed that recombinant isoforms with different epitope structure can focus under the same protein spot after electrophoresis. The structure of conformational epitopes can be distorted by amino acid substitutions even when T-cell epitopes are not affected as judged by T-cell proliferation studies.
引用
收藏
页码：251 / 254
页数：4
相关论文
共 50 条
  • [1] Structural characterisation of a recombinant Bet v 1 allergen established as a safe and efficacious treatment for birch pollen allergy
    Nony, E.
    Le Mignon, M.
    Lemoine, P.
    Jain, K.
    Didierlaurent, A.
    Batard, T.
    Moingeon, P.
    ALLERGY, 2014, 69 : 561 - 561
  • [2] Characterisation of recombinant Bet v 1 as a candidate vaccine against birch pollen allergy
    Nony, E.
    Chabre, H.
    Lautrette, A.
    Lemoine, P.
    Couret, M.
    Bobinnec, Y.
    Didierlaurent, A.
    Batard, T.
    Moingeon, P.
    ALLERGY, 2008, 63 : 232 - 232
  • [3] Bioavailability of the major birch pollen allergen Bet v 1
    Ochs, S
    Wallmuth, A
    Kasche, A
    Weichenmeier, I
    Traidl-Hoffmann, C
    Huss-Marp, J
    Krämer, U
    Link, E
    Gebauer, G
    Schober, W
    Behrendt, H
    Buters, JTM
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2006, 372 : 134 - 134
  • [4] Skin response to recombinant birch pollen allergen (Bet v1a) in allergic patients during and out of the birch pollen season
    Brzezinska-Pawlowska, O.
    Lupinek, C.
    Piechota-Polanczyk, A.
    Kurowski, M.
    Jablonska, A.
    Stolz, F.
    Kopp, S.
    Neubauer, A.
    Kiss, R.
    Valenta, R.
    Kowalski, M. L.
    ALLERGY, 2015, 70 : 173 - 173
  • [5] Determination of nitration degrees for the birch pollen allergen Bet v 1
    Kathrin Selzle
    Chloé Ackaert
    Christopher J. Kampf
    Anna T. Kunert
    Albert Duschl
    Gertie J. Oostingh
    Ulrich Pöschl
    Analytical and Bioanalytical Chemistry, 2013, 405 : 8945 - 8949
  • [6] The History and Science of the Major Birch Pollen Allergen Bet v 1
    Breiteneder, Heimo
    Kraft, Dietrich
    BIOMOLECULES, 2023, 13 (07)
  • [7] Determination of nitration degrees for the birch pollen allergen Bet v 1
    Selzle, Kathrin
    Ackaert, Chloe
    Kampf, Christopher J.
    Kunert, Anna T.
    Duschl, Albert
    Oostingh, Gertie J.
    Poeschl, Ulrich
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (27) : 8945 - 8949
  • [8] Seven different genes encode a diverse mixture of isoforms of Bet v 1, the major birch pollen allergen
    Martijn F Schenk
    Ludovicus JWJ Gilissen
    Gerhard D Esselink
    Marinus JM Smulders
    BMC Genomics, 7
  • [9] Serological and skin-test diagnosis of birch pollen allergy with recombinant Bet v I, the major birch pollen allergen
    Menz, G
    Dolecek, C
    SchonheitKenn, U
    Ferreira, F
    Moser, M
    Schneider, T
    Suter, M
    BoltzNitulescu, G
    Ebner, C
    Kraft, D
    Valenta, R
    CLINICAL AND EXPERIMENTAL ALLERGY, 1996, 26 (01): : 50 - 60
  • [10] Recombinant Bet v 1 vaccine for treatment of allergy to birch pollen
    Gronlund, Hans
    Gafvelin, Guro
    HUMAN VACCINES, 2010, 6 (12): : 970 - 977
12345