Degradable and Bioadhesive Alginate-Based Composites: An Effective Hemostatic Agent

被引:61
作者
Huang, He [1 ,2 ,5 ,6 ]
Chen, Hongsai [1 ,2 ,3 ,5 ]
Wang, Xueling [1 ,2 ,5 ]
Qiu, Fangxia [7 ]
Liu, Huihui [1 ,2 ,5 ]
Lu, Jiawen [1 ,2 ,5 ]
Tong, Ling [1 ,2 ,5 ]
Yang, Yuming [7 ]
Wang, Xiansong [4 ]
Wu, Hao [1 ,2 ,5 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Otolaryngol Head & Neck Surg, Shanghai 200011, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Ear Inst, Shanghai 200011, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Shanghai Inst Precis Med, Shanghai 200011, Peoples R China
[4] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Key Lab Tissue Engn, Sch Med,Natl Tissue Engn Ctr China, Shanghai 200011, Peoples R China
[5] Shanghai Key Lab Translat Med Ear & Nose Dis, Shanghai 200125, Peoples R China
[6] Anhui Med Univ, Affiliated Hosp 1, Dept Plast & Reconstruct Surg, Hefei 230032, Anhui, Peoples R China
[7] Zhejiang Sanchuang Biotechnol Co Ltd, Jiaxing 314031, Zhejiang, Peoples R China
来源
ACS BIOMATERIALS SCIENCE & ENGINEERING | 2019年 / 5卷 / 10期
基金
中国国家自然科学基金;
关键词
hemostasis; degradable alginate-based composites; biocompatibility; in vitro; in vivo animal model; CALCIUM-ENTRY; BLOOD; MORTALITY; COLLAGEN; SPONGE;
D O I
10.1021/acsbiomaterials.9b01120
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
The perfect hemostatic material should be capable of rapidly controlling substantial hemorrhaging from visceral organs, veins, and arteries. Ideally, it should be biodegradable, biocompatible, easily applied, and inexpensive. Herein, taking advantages of sodium alginate (SA), carboxymethyl chitosan (CMC), and collagen, a degradable powdery hemostatic composite (SACC) was synthesized using emulsification and cross-linking technology. The morphology and structure of SACC were determined using Fourier transform infrared spectroscopy and scanning electron microscopy (SEM). This hemostatic material exhibited a typical generic sphere shape with narrow size distribution, rough surface, and satisfactory water absorption. Using in vitro bleeding and in vivo bleeding models (rat liver injury model and rat tail amputation model), it was shown that SACC had superior hemostatic actions compared to CMC and SA. Excellent cytocompatibility was proven during cytotoxicity tests and SEM observations. Histomorphological evaluation during the wound healing process proved the superior biocompatibility of SACC in a rat liver injury model. Biodegradability of SACC was demonstrated by immunofluorescence techniques both in vitro and in vivo. In summary, we have demonstrated the enormous potential of SACC, which has excellent hemostatic activity, biodegradability, and biocompatibility properties for use in clinical hemostasis applications.
引用
收藏
页码:5498 / +
页数:15
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