Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches

被引：15
|
作者：
Liu, Wenjing [1 ,2 ]
Guo, Yourong [3 ]
Wang, Kaizhe [1 ,2 ]
Zhou, Xingfei [4 ]
Wang, Ying [1 ,5 ]
Lu, Junhong [1 ,5 ]
Shao, Zhifeng [3 ]
Hu, Jun [1 ,5 ,6 ]
Czajkowsky, Daniel M. [3 ]
Li, Bin [1 ,5 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Biomed Engn, BioID Ctr, Shanghai 200240, Peoples R China
[4] Ningbo Univ, Sch Sci, Ningbo 315211, Zhejiang, Peoples R China
[5] Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai 201210, Peoples R China
[6] Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201204, Peoples R China
来源：
NANOSCALE | 2019年 / 11卷 / 37期
基金：
中国国家自然科学基金;
关键词：
RESOLUTION; RECOGNITION; MECHANICS; PROTEINS;
D O I：
10.1039/c9nr05234h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.
引用
收藏
页码：17206 / 17210
页数：5
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