Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches

被引：15
|
作者：
Liu, Wenjing [1 ,2 ]
Guo, Yourong [3 ]
Wang, Kaizhe [1 ,2 ]
Zhou, Xingfei [4 ]
Wang, Ying [1 ,5 ]
Lu, Junhong [1 ,5 ]
Shao, Zhifeng [3 ]
Hu, Jun [1 ,5 ,6 ]
Czajkowsky, Daniel M. [3 ]
Li, Bin [1 ,5 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Biomed Engn, BioID Ctr, Shanghai 200240, Peoples R China
[4] Ningbo Univ, Sch Sci, Ningbo 315211, Zhejiang, Peoples R China
[5] Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai 201210, Peoples R China
[6] Shanghai Tech Univ, Sch Phys Sci & Technol, Shanghai 201204, Peoples R China
来源：
NANOSCALE | 2019年 / 11卷 / 37期
基金：
中国国家自然科学基金;
关键词：
RESOLUTION; RECOGNITION; MECHANICS; PROTEINS;
D O I：
10.1039/c9nr05234h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.
引用
收藏
页码：17206 / 17210
页数：5
相关论文
共 50 条
  • [1] Going Vertical To Improve the Accuracy of Atomic Force Microscopy Based Single-Molecule Force Spectroscopy
    Walder, Robert
    Van Patten, William J.
    Adhikari, Ayush
    Perkins, Thomas T.
    ACS NANO, 2018, 12 (01) : 198 - 207
  • [2] Application of Atomic Force Microscopy (AFM)-based Single-molecule Force Spectroscopy (SMFS) in Polymer Characterization
    Zhang, Wei
    Hou, Jue
    Li, Nan
    Zhang, Wen-ke
    ACTA POLYMERICA SINICA, 2021, 52 (11): : 1523 - 1546
  • [3] Atomic force microscopy-based force spectroscopy u biological and biomedical applications
    Carvalho, Filomena A.
    Santos, Nuno C.
    IUBMB LIFE, 2012, 64 (06) : 465 - 472
  • [4] Open the door to the atomic world by single-molecule atomic force microscopy
    Fang, Siyuan
    Hu, Yun Hang
    MATTER, 2021, 4 (04) : 1189 - 1223
  • [5] Nanomechanical DNA origami 'single-molecule beacons' directly imaged by atomic force microscopy
    Kuzuya, Akinori
    Sakai, Yusuke
    Yamazaki, Takahiro
    Xu, Yan
    Komiyama, Makoto
    NATURE COMMUNICATIONS, 2011, 2
  • [6] Molecular Processes Studied at a Single-Molecule Level Using DNA Origami Nanostructures and Atomic Force Microscopy
    Bald, Ilko
    Keller, Adrian
    MOLECULES, 2014, 19 (09) : 13803 - 13823
  • [7] Single-molecule switching with non-contact atomic force microscopy
    Schuette, Jens
    Bechstein, Ralf
    Rahe, Philipp
    Langhals, Heinz
    Rohlfing, Michael
    Kuehnle, Angelika
    NANOTECHNOLOGY, 2011, 22 (24)
  • [8] Thermal denaturation of fibrinogen visualized by single-molecule atomic force microscopy
    Barinov, Nikolay A.
    Protopopova, Anna D.
    Dubrovin, Evgeniy V.
    Klinov, Dmitry V.
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2018, 167 : 370 - 376
  • [9] Single-molecule imaging of DNA polymerase I (Klenow fragment) activity by atomic force microscopy
    Chao, J.
    Zhang, P.
    Wang, Q.
    Wu, N.
    Zhang, F.
    Hu, J.
    Fan, C. H.
    Li, B.
    NANOSCALE, 2016, 8 (11) : 5842 - 5846
  • [10] Study of PNA-DNA hybridization by AFM-based single-molecule force spectroscopy
    Cao, Meiwen
    Deng, Li
    Xu, Hai
    COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2015, 470 : 46 - 51
12345