Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis

被引：133
|
作者：
Vasmatzis, G [1 ]
Essand, M [1 ]
Brinkmann, U [1 ]
Lee, B [1 ]
Pastan, I [1 ]
机构：
[1] NCI, Mol Biol Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 01期
关键词：
D O I：
10.1073/pnas.95.1.300
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A procedure is described to discover genes that are specifically expressed in human prostate, The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries, The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern blots to examine the transcript size of the corresponding mRNA. The computer analysis identified 15 promising genes that were previously unidentified. When seven of these were examined in an RNA hybridization experiment, three were found to be prostate specific, The genes identified could be useful in the targeted therapy of prostate cancer. The procedure can easily be applied to discover genes specifically expressed in other organs or tumors.
引用
收藏
页码：300 / 304
页数：5
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