Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples

被引：13

作者：

Watanabe, Ryuichi ^{[1
]}

Kanamori, Makoto ^{[2
]}

Yoshida, Hidetsugu ^{[2
]}

Okumura, Yutaka ^{[3
]}

Uchida, Hajime ^{[1
]}

Matsushima, Ryoji ^{[1
]}

Oikawa, Hiroshi ^{[1
]}

Suzuki, Toshiyuki ^{[1
]}

机构：

[1] Natl Res Inst Fisheries Sci, Kanazawa Ku, Fukuura 2-12-4, Yokohama, Kanagawa 2368648, Japan

[2] Hakodate Fisheries Res Inst, Hokkaido Res Org, Benten Cho 20-5, Hakodate, Hokkaido 0400051, Japan

[3] Tohoku Natl Fisheries Res Inst, Shinhama Cho 3-27-5, Shiogama, Miyagi 9850001, Japan

来源：

TOXINS | 2019年 / 11卷 / 10期

关键词：

saxitoxin analogues; UPLC; shellfish toxicity; OX; FD; post-column detection; saxitoxin; PARALYTIC SHELLFISH TOXINS; CRABS TELMESSUS-ACUTIDENS; POISONING TOXINS; CHARYBDIS-JAPONICA; ACCUMULATION;

D O I：

10.3390/toxins11100573

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Saxitoxin (STX) and its analogues produced by toxic dinoflagellates accumulate in bivalves, and routine monitoring of bivalves is important to prevent cases of human poisoning. In this study, we describe a rapid detection method for the analysis of STXs using ultra-performance liquid chromatography with post-column fluorescent detection and to investigate water depths and sampling points optimal for shellfish toxin monitoring. Cultured scallops (Mizuhopecten yessoensis) and mussels (Mytilus galloprovincialis) collected from various water depths and sampling points were used in this study. Irrespective of bivalve species, toxin concentrations in bivalves were lower at deeper water depths. The toxin concentrations of bivalves did not differ greatly when bivalves were collected from the same bay. Although the levels of contamination of bivalves with STXs can depend on various environmental and geographical factors, our findings are useful for formulating a sampling protocol for the prevention of harvesting contaminated shellfish.

引用

页数：11

共 22 条

[1]

Anon A., 2005, 200506 AOAC INT

[2]

Anon A., 2011, 201102 AOAC INT

[3]

AOAC, 2005, AOAC OFF METHODS ANA, P79

[4] Development of a sensitive and selective liquid chromatography-mass spectrometry method for high throughput analysis of paralytic shellfish toxins using graphitised carbon solid phase extraction [J].

Boundy, Michael J. ;

Selwood, Andrew I. ;

Harwood, D. Tim ;

McNabb, Paul S. ;

Turner, Andrew D. .

JOURNAL OF CHROMATOGRAPHY A, 2015, 1387 :1-12

[5] Voltage-gated ion channels and gating modifier toxins [J].

Catterall, William A. ;

Cestele, Sandrine ;

Yarov-Yarovoy, Vladimir ;

Yu, Frank H. ;

Konoki, Keiichi ;

Scheuer, Todd .

TOXICON, 2007, 49 (02) :124-141

[6] Saxitoxin Exposure Confirmed by Human Urine and Food Analysis [J].

Coleman, R. M. ;

Ojeda-Torres, G. ;

Bragg, W. ;

Fearey, D. ;

McKinney, P. ;

Castrodale, L. ;

Verbrugge, D. ;

Stryker, K. ;

DeHart, E. ;

Cooper, M. ;

Hamelin, E. ;

Thomas, J. ;

Johnson, R. C. .

JOURNAL OF ANALYTICAL TOXICOLOGY, 2018, 42 (07) :E61-E64

[7] Non-traditional vectors for paralytic shellfish poisoning [J].

Deeds, Jonathan R. ;

Landsberg, Jan H. ;

Etheridge, Stacey M. ;

Pitcher, Grant C. ;

Longan, Sara Watt .

MARINE DRUGS, 2008, 6 (02) :308-348

[8] Paralytic shellfish toxins in clinical matrices: Extension of AOAC official method 2005.06 to human urine and serum and application to a 2007 case study in Maine [J].

DeGrasse, Stacey ;

Rivera, Victor ;

Roach, John ;

White, Kevin ;

Callahan, John ;

Couture, Darcie ;

Simone, Karen ;

Peredy, Tamas ;

Poli, Mark .

DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 2014, 103 :368-375

[9] Occurrence and toxin production of Alexandrium spp. (Dinophyceae) in coastal waters of Iwate Prefecture, Japan [J].

Kaga, Shinnosuke ;

Sekiguchi, Katsushi ;

Yoshida, Makoto ;

Ogata, Takehiko .

NIPPON SUISAN GAKKAISHI, 2006, 72 (06) :1068-1076

[10]

Lawrence J., 2011, The FAO Fisheries and Aquaculture Technical Papers, V551, P274

← 1 2 3 →