Development of Immunochromatographic Test Kit for Rapid Detection of Specific IgG4 Antibody in Whole-Blood Samples for Diagnosis of Human Gnathostomiasis

被引：9

作者：

Janwan, Penchom ^{[1
]}

Intapan, Pewpan M. ^{[2
,3
]}

Sadaow, Lakkhana ^{[2
,3
]}

Rodpai, Rutchanee ^{[2
,3
]}

Yamasaki, Hiroshi ^{[4
]}

Boonroumkaew, Patcharaporn ^{[2
,3
]}

Sanpool, Oranuch ^{[2
,3
]}

Thanchomnang, Tongjit ^{[3
,5
]}

Sadee, Phuangphaka ^{[6
]}

Maleewong, Wanchai ^{[2
,3
]}

机构：

[1] Walailak Univ, Sch Allied Hlth Sci, Dept Med Technol, Nakhon Si Thammarat 80161, Thailand

[2] Khon Kaen Univ, Fac Med, Dept Parasitol, Khon Kaen 40002, Thailand

[3] Khon Kaen Univ, Mekong Hlth Sci Res Inst, Khon Kaen 40002, Thailand

[4] Natl Inst Infect Dis, Dept Parasitol, Tokyo 1628640, Japan

[5] Mahasarakham Univ, Fac Med, Maha Sarakham 44000, Thailand

[6] Khon Kaen Univ, Srinagarind Hosp, Fac Med, Clin Immunol Unit, Khon Kaen 40002, Thailand

来源：

DIAGNOSTICS | 2021年 / 11卷 / 05期

关键词：

human gnathostomiasis; immunochromatographic test kit; point-of-care test; whole-blood sample; serodiagnosis; IgG4; antibody; MATRIX-METALLOPROTEINASE PROTEIN; SPINIGERUM; SERODIAGNOSIS; IMMUNODIAGNOSIS; ANTIGEN; ELISA; HELMINTHS;

D O I：

10.3390/diagnostics11050862

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Human gnathostomiasis is a harmful food-borne zoonosis caused by roundworms of the genus Gnathostoma. The parasite can occasionally migrate to the central nervous system, causing life-threatening disease and death. Here, we report a new point-of-care (POC) test kit, the gnathostomiasis blood immunochromatographic test (GB-ICT) kit. The kit is based on recombinant Gnathostoma spinigerum antigen and detects specific IgG4 antibody in whole-blood samples (WBSs). The GB-ICT kit showed potentially high diagnostic values with simulated WBSs (n = 248), which were obtained by spiking patients' sera with red blood cells. The accuracy, sensitivity, specificity, and positive and negative predictive values were 95.2%, 100%, 93.8%, 81.5%, and 100%, respectively. Ten WBSs from clinically suspected gnathostomiasis patients were all positive according to the GB-ICT kit, while 10 WBSs from healthy volunteers were negative. The GB-ICT kit is a simple and convenient POC testing tool using finger-prick blood samples: venous blood sampling and serum separation processes are not required. The GB-ICT kit can support clinical diagnosis in remote areas and field settings without sophisticated equipment facilities.

引用

页数：10

共 43 条

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