Domain disruptions of individual 3B proteins of foot-and-mouth disease virus do not alter growth in cell culture or virulence in cattle

被引：24

作者：

Pacheco, Juan M. ^{[1
]}

Piccone, Maria E. ^{[1
,2
]}

Rieder, Elizabeth ^{[1
]}

Pauszek, Steven J. ^{[1
]}

Borca, Manuel V. ^{[1
]}

Rodriguez, Luis L. ^{[1
]}

机构：

[1] ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY USA

[2] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT USA

来源：

VIROLOGY | 2010年 / 405卷 / 01期

关键词：

Foot-and-mouth disease; Protein; 38; Mutagenesis; Cattle; GENOME-LINKED PROTEIN; NONSTRUCTURAL PROTEINS; RNA-POLYMERASE; IN-VITRO; POLIOVIRUS; GENE; VPG; REPLICATION; MUTANTS; 3A;

D O I：

10.1016/j.virol.2010.05.036

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Picornavirus RNA replication is initiated by a small viral protein primer, 38 (also known as VPg). that is covalently linked to the 5' terminus of the viral genome. In contrast to other picornaviruses that encode a single copy of 38, foot-and-mouth disease virus (FMDV) encodes three copies of 38 Viruses containing disrupted native sequence or deletion of one of their three 38 proteins were derived from a FMDV A24 Cruzeiro full-length cDNA infectious clone Mutant viruses had growth characteristics similar to the parental virus in cells. RNA synthesis and protein cleavage processes were not significantly affected in these mutant viruses Cattle infected by aerosol exposure with mutant viruses developed clinical disease similar to that caused by the parental A24 Cruzeiro. Therefore, severe domain disruption or deletion of individual 38 proteins in FMDV do not affect the virus' ability to replicate in vitro and cause clinical disease in cattle Published by Elsevier Inc

引用

页码：149 / 156

页数：8

共 34 条

[1] The pathogenesis and diagnosis of foot-and-mouth disease [J].

Alexandersen, S ;

Zhang, Z ;

Donaldson, AI ;

Garland, AJM .

JOURNAL OF COMPARATIVE PATHOLOGY, 2003, 129 (01) :1-36

[2] Use of a portable real-time reverse transcriptase-polymerase chain reaction assay for rapid detection of foot-and-mouth disease virus [J].

Callahan, JD ;

Brown, F ;

Csorio, FA ;

Sur, JH ;

Kramer, E ;

Long, GW ;

Lubroth, J ;

Ellis, SJ ;

Shoulars, KS ;

Gaffney, KL ;

Rock, DL ;

Nelson, WM .

JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 2002, 220 (11) :1636-1642

[3] Genetic and phenotypic variation of foot-and-mouth disease virus during serial passages in a natural host [J].

Carrillo, C. ;

Lu, Z. ;

Borca, M. V. ;

Vagnozzi, A. ;

Kutish, G. F. ;

Rock, D. L. .

JOURNAL OF VIROLOGY, 2007, 81 (20) :11341-11351

[4]

DE CASTRO M. P., 1964, ARQ INST BIOL [SAO PAULO], V31, P63

[5] Interactions of foot-and-mouth disease virus with soluble bovine αVβ3 and αVβ6 integrins [J].

Duque, H ;

LaRocco, M ;

Golde, WT ;

Baxt, B .

JOURNAL OF VIROLOGY, 2004, 78 (18) :9773-9781

[6] VPG-GENE AMPLIFICATION CORRELATES WITH INFECTIVE PARTICLE FORMATION IN FOOT-AND-MOUTH-DISEASE VIRUS [J].

FALK, MM ;

SOBRINO, F ;

BECK, E .

JOURNAL OF VIROLOGY, 1992, 66 (04) :2251-2260

[7] A TANDEM REPEAT GENE IN A PICORNAVIRUS [J].

FORSS, S ;

SCHALLER, H .

NUCLEIC ACIDS RESEARCH, 1982, 10 (20) :6441-6450

[8] Differential distribution of non-structural proteins of foot-and-mouth disease virus in BHK-21 cells [J].

Garcia-Briones, Mercedes ;

Rosas, Maria F. ;

Gonzalez-Magaldi, Monica ;

Martin-Acebes, Miguel A. ;

Sobrino, Francisco ;

Armas-Portela, Rosario .

VIROLOGY, 2006, 349 (02) :409-421

[9] Vaccination against foot-and-mouth disease virus confers complete clinical protection in 7 days and partial protection in 4 days: Use in emergency outbreak response [J].

Golde, WT ;

Pacheco, JM ;

Duque, H ;

Doel, T ;

Penfold, B ;

Ferman, GS ;

Gregg, DR ;

Rodriguez, LL .

VACCINE, 2005, 23 (50) :5775-5782

[10] Foot-and-mouth disease [J].

Grubman, MJ ;

Baxt, B .

CLINICAL MICROBIOLOGY REVIEWS, 2004, 17 (02) :465-+

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