Purification and structural elucidation of a water-soluble polysaccharide from the fruiting bodies of the Grifola frondosa

被引：42

作者：

Zhang, Anqiang ^{[1
]}

Deng, Jiaying ^{[1
]}

Yu, Shuying ^{[1
]}

Zhang, Fuming ^{[2
,3
,4
]}

Linhardt, Robert J. ^{[2
,3
,4
]}

Sun, Peilong ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Dept Food Sci & Technol, Hangzhou 310014, Zhejiang, Peoples R China

[2] Rensselaer Polytech Inst, Dept Chem & Chem Biol, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[3] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Biol Sci & Biomed Engn, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[4] Rensselaer Polytech Inst, Dept Biol Sci & Biomed Engn, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 115卷

关键词：

Grifola frondosa; Polysaccharide; Purification; Structural elucidation; ACID;

D O I：

10.1016/j.ijbiomac.2018.04.061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Grifola frondosa is a polypore mushroom, which has been used for many centuries by traditional Chinese and Japanese herbalists as a medicinal mushroom. A water-soluble polysaccharide (code as GFP30-2-a, molecular weight: 2.04 x 10(6) Da) was isolated from the fruiting bodies of G. frondosa by hot water extraction, ethanol precipitation and chromatography. Its structure was elucidated from its monosaccharide composition, methylation analysis, together with 1D NMR (H-1 NMR and C-13 NMR) and 2D NMR (COSY, TOCSY, HSQC, HMBC and NOESY spectra). GFP30-2-a consists of D-Glc and D-Gal in the molar ratio of 1:0.098 and the structure of the repeating units was identified to be beta D Glcp (1 -> [4) alpha D Glcp (1 -> 4) alpha D Glcp (1](m) -> 4) alpha D Glcp. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：221 / 226

页数：6

共 13 条

[1] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].