Evidence for covalent binding of epicocconone with proteins from synchronous fluorescence spectra and fluorescence lifetimes

被引：9

作者：

Panda, Debashis ^{[1
]}

Datta, Anindya ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Bombay 400076, Maharashtra, India

来源：

JOURNAL OF CHEMICAL SCIENCES | 2007年 / 119卷 / 02期

关键词：

covalent binding; epicocconone; synchronous fluorescence spectra; fluorescence lifetimes;

D O I：

10.1007/s12039-007-0016-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Synchronous fluorescence and time-resolved fluorescence spectroscopic studies that reveal the interaction of epicocconone with human serum albumin is significantly different from its interaction with surfactant assemblies. This observation, along with steady-state fluorescence data, indicates groundstate interaction between the fluorophore epicocconone and the protein. Similarity in fluorescence properties with the adduct of the fluorophore with n-butylamine indicates that bonding occurs at the N-terminus of the protein.

引用

页码：99 / 104

页数：6

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