Synthesis of Lysophospholipids

被引:112
作者
D'Arrigo, Paola [1 ,2 ,3 ]
Servi, Stefano [1 ,2 ,3 ]
机构
[1] Politecn Milan, Dipartimento Chim Mat & Ingn Chim Giulio Natta, I-20131 Milan, Italy
[2] Politecn Milan, Ctr Interuniv Ric Biotecnol Prote, Prot Factory, I-20131 Milan, Italy
[3] Univ Insubria, I-20131 Milan, Italy
关键词
lysophospholipids; biocatalysis; phospholipase; chemo-enzymatic synthesis; lysophosphatidylcholine; lysophosphatidic acid; POLYUNSATURATED FATTY-ACID; LIPASE-CATALYZED SYNTHESIS; TANDEM MASS-SPECTROMETRY; OVARIAN-CANCER CELLS; PHOSPHOLIPASE-D; LYSOPHOSPHATIDIC ACID; SUBSTRATE-SPECIFICITY; POTENTIAL BIOMARKERS; ENZYMATIC-HYDROLYSIS; IMMOBILIZED LIPASE;
D O I
10.3390/molecules15031354
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
New synthetic methods for the preparation of biologically active phospholipids and lysophospholipids (LPLs) are very important in solving problems of membrane-chemistry and biochemistry. Traditionally considered just as second-messenger molecules regulating intracellular signalling pathways, LPLs have recently shown to be involved in many physiological and pathological processes such as inflammation, reproduction, angiogenesis, tumorogenesis, atherosclerosis and nervous system regulation. Elucidation of the mechanistic details involved in the enzymological, cell-biological and membrane-biophysical roles of LPLs relies obviously on the availability of structurally diverse compounds. A variety of chemical and enzymatic routes have been reported in the literature for the synthesis of LPLs: the enzymatic transformation of natural glycerophospholipids (GPLs) using regiospecific enzymes such as phospholipases A(1) (PLA(1)), A(2) (PLA(2)) phospholipase D (PLD) and different lipases, the coupling of enzymatic processes with chemical transformations, the complete chemical synthesis of LPLs starting from glycerol or derivatives. In this review, chemo-enzymatic procedures leading to 1-and 2-LPLs will be described.
引用
收藏
页码:1354 / 1377
页数:24
相关论文
共 105 条