Gold nanoparticle-based colorimetric assay for cancer diagnosis

被引：56

作者：

Kang, Jeong-Hun ^{[1
]}

Asami, Yoji ^{[2
]}

Murata, Masaharu ^{[3
]}

Kitazaki, Hirotaro ^{[2
,4
]}

Sadanaga, Noriaki ^{[4
]}

Tokunaga, Erik ^{[4
]}

Shiotani, Satoko ^{[4
]}

Okada, Satoko ^{[4
]}

Maehara, Yoshihiko ^{[4
]}

Niidome, Takuro ^{[1
,2
,5
]}

Hashizume, Makoto ^{[3
]}

Mori, Takeshi ^{[1
,2
,5
]}

Katayama, Yoshiki ^{[1
,2
,5
]}

机构：

[1] Kyushu Univ, Fac Engn, Dept Appl Chem, Nishi Ku, Fukuoka 8190395, Japan

[2] Kyushu Univ, Grad Sch Syst Life Sci, Nishi Ku, Fukuoka 8190395, Japan

[3] Kyushu Univ, Dept Adv Med Initiat, Fac Med Sci, Higashi Ku, Fukuoka 8128582, Japan

[4] Kyushu Univ, Dept Surg & Sci, Grad Sch Med Sci, Higashi Ku, Fukuoka 8128582, Japan

[5] Kyushu Univ, Ctr Future Chem, Nishi Ku, Fukuoka 8190395, Japan

来源：

BIOSENSORS & BIOELECTRONICS | 2010年 / 25卷 / 08期

关键词：

Protein kinase; Gold nanoparticle; Diagnosis; Phosphorylation; Peptide; PROTEIN-KINASE-C; MULTIDRUG-RESISTANCE; ALPHA EXPRESSION; BREAST-TISSUE; CHO CELLS; PKC-ALPHA; AGGREGATION; ACTIVATION; RECEPTORS; SUBSTRATE;

D O I：

10.1016/j.bios.2009.12.022

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel gold nanoparticle (GNP)-based colorimetric assay was developed for cancer diagnosis. This system is based on the noncrosslinking aggregation mechanism with a cationic protein kinase C (PKC) alpha-specific peptide substrate, which is used as a coagulant of citrate-coated GNP with anionic surface charges. The phosphorylation of peptide substrates by PKC alpha suppressed GNP aggregation, resulting in a red color, but in the case of non-phosphorylation, the color of the GNP solution changed from red to blue, indicating particle aggregation. Moreover, a correlation between the color change of the GNP dispersions and the level of activated PKC alpha was identified from experiments using cancer cell lines, or xenografted mouse cancer and normal mouse tissues. When our system was applied to human breast cancers and normal human breast tissues, cancer tissue lysates became red in color, indicating GNP dispersion, while all lysates from normal tissue turned the GNP solution blue. MALDI-TOF MS analysis and Western blotting experiment confirmed that these different results between cancer and normal tissues reflected the difference in PKC alpha activity. This study is the first report on the application of the GNP-based colorimetric assay to the diagnosis of cancer. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：1869 / 1874

页数：6

共 42 条

[1]

AHSEN O, 2005, CHEMBIOCHEM, V6, P481

[2]

BLOBE GC, 1993, J BIOL CHEM, V268, P658

[3]

Chatterjee Sabarni K, 2005, Future Oncol, V1, P37, DOI 10.1517/14796694.1.1.37

[4] Biosensors of protein kinase action: from in vitro assays to living cells [J].