A novel er1 allele and the development and validation of its functional marker for breeding pea (Pisum sativum L.) resistance to powdery mildew

被引:24
|
作者
Sun, Suli [1 ]
Deng, Dong [1 ]
Wang, Zhongyi [1 ]
Duan, Canxing [1 ]
Wu, Xiaofei [1 ]
Wang, Xiaoming [1 ]
Zong, Xuxiao [1 ]
Zhu, Zhendong [1 ]
机构
[1] Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Inst Crop Sci, 12 Zhongguancun South St, Beijing 100081, Peoples R China
关键词
ERYSIPHE-PISI; CONFERRING RESISTANCE; SCAR MARKERS; GENE; IDENTIFICATION; RAPD; LINKAGE; ER-1; POLYMORPHISM; GENOTYPES;
D O I
10.1007/s00122-016-2671-9
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Key message A novel er1 allele, er1 -7, conferring pea powdery mildew resistance was characterized by a 10-bp deletion in PsMLO1 cDNA, and its functional marker was developed and validated in pea germplasms. Pea powdery mildew caused by Erysiphe pisi DC is a major disease worldwide. Pea cultivar 'DDR-11' is an elite germplasm resistant to E. pisi. To identify the gene conferring resistance in DDR-11, the susceptible Bawan 6 and resistant DDR-11 cultivars were crossed to produce F-1, F-2, and F-2:3 populations. The phenotypic segregation patterns in the F-2 and F-2:3 populations fit the 3:1 (susceptible:resistant) and 1:2:1 (susceptible homozygotes:heterozygotes:resistant homozygotes) ratios, respectively, indicating that resistance was controlled by a single recessive gene. Analysis of er1-linked markers in the F-2 population suggested that the recessive resistance gene in DDR-11 was an er1 allele, which was mapped between markers ScOPE16-1600 and c5DNAmet. To further characterize er1 allele, the cDNA sequences of PsMLO1 from the parents were obtained and a novel er1 allele in DDR-11 was identified and designated as er1-7, which has a 10-bp deletion in position 111-120. The er1-7 allele caused a frame-shift mutation, resulting in a premature termination of translation of PsMLO1 protein. A co-dominant functional marker specific for er1-7 was developed, InDel111-120, which co-segregated with E. pisi resistance in the mapping population. The marker was able to distinguish between pea germplasms with and without the er1-7. Of 161 pea germplasms tested by InDel111-120, seven were detected containing resistance allele er1-7, which was verified by sequencing their PsMLO1 cDNA. Here, a novel er1 allele was characterized and its an ideal functional marker was validated, providing valuable genetic information and a powerful tool for breeding pea resistance to powdery mildew.
引用
收藏
页码:909 / 919
页数:11
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