Gene land protein profiling of the response of MA-10 Leydig tumor cells to human chorionic gonadotropin

被引:0
作者
Li, WP
Amri, H
Huang, HZ
Wu, C
Papadopoulos, V
机构
[1] Georgetown Univ, Dept Biochem & Mol Biol, Ctr Med, Washington, DC 20057 USA
[2] Georgetown Univ, Dept Physiol & Biophys, Ctr Med, Washington, DC 20057 USA
[3] Georgetown Univ, Dept Prot Informat Resource, Ctr Med, Washington, DC 20057 USA
来源
JOURNAL OF ANDROLOGY | 2004年 / 25卷 / 06期
关键词
testis; gene ontology; genomics; proteomics; steroidogenesis;
D O I
暂无
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
Activation of the steroidogenic machinery by peptide hormones involves a number of steps for transmitting signals from the plasma membrane to mitochondria in a spatially and temporally coordinated manner. Although key. proteins mediating the hormonal signal have been identified, recent data suggest that the pathway might involve more complex protein-protein and protein-lipid interactions. Genomic and proteomic methods of analysis, namely the Affymetrix Murine Genome U74A v2 GeneChip and the BD PowerBlot Western Array, were used to identify human chorionic gonadotropin (hCG)-induced changes in mRNA and protein of MA-10 Leydig tumor cells that parallel the increase seen in progesterone synthesis. To analyze the massive amount of data that was generated, a comprehensive protein information matrix summarizing the features of each gene or protein, including its known properties, as well as annotations derived by homology-based functional inference, Was developed. Of the genes examined by Affymetrix array, approximately. 79 were differentially expressed and of gene products examined by PowerBlot, 9 were differentially expressed (above twofold). Changes in the expression of selected transcripts of interest were confirmed using real-time quantitative polymerase chain reaction and immunoblot analyses. Collectively, these results indicate that hormonal regulation of steroidogenesis is a complex phenomenon, involving proteins that participate in various known and novel pathways, which are implicated in transmitting signals from the plasma membrane to mitochondria and nucleus.
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页码:900 / 913
页数:14
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