SERS Assay for Copper(II) Ions Based on Dual Hot-Spot Model Coupling with MarR Protein: New Cu2+-Specific Biorecognition Element

被引：32

作者：

Wang, Yulong ^{[1
]}

Su, Zhenhe ^{[1
]}

Wang, Limin ^{[1
]}

Dong, Jinbo ^{[1
]}

Xue, Juanjuan ^{[1
]}

Yu, Jiao ^{[1
]}

Wang, Yuan ^{[1
]}

Hua, Xiude ^{[1
]}

Wang, Minghua ^{[1
]}

Zhang, Cunzheng ^{[2
]}

Liu, Fengquan ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 12期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

FUNCTIONALIZED GOLD NANOPARTICLES; COLORIMETRIC DETECTION; SENSORS; METAL; AG; RECOGNITION; ABSORPTION; BIOSENSOR; MERCURY; BINDING;

D O I：

10.1021/acs.analchem.6b05106

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed a rapid and ultrasensitive surface-enhanced Raman scattering (SERS) assay for Cu2+ detection using the multiple antibiotic resistance regulator (MarR) as specific bridging molecules in a SERS hot-spot model. In the assay, Cu2+ induces formation of MarR tetramers, which provide Au nanoparticle (NP) AuNP bridges, resulting in the formation of SERS hot spots. 4-Mercaptobenzoic acid (4-MBA) was used as a Raman reporter. The addition of Cu2+ increased the Raman intensity of 4-MBA. Use of a dual hot-spot signal-amplification strategy based on AuNP-AgNP heterodimers combined through antigen antibody reactions increased the sensitivity of the sensing platform by 50-fold. The proposed method gave a linear response for Cu2+ detection in the range of 0.5-1000 nM, with a detection limit of 0.18 nM, which is 5 orders of magnitude lower than the U.S. Environmental Protection Agency limit for Cu2+ in drinking water (20 mu M). In addition, all analyses can be completed in less than 15 min. The high sensitivity, high specificity, and rapid detection capacity of the SERS assay therefore provide a combined advantage over current assays.

引用

页码：6392 / 6398

页数：7